Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACSM5 protein (Right) or empty vector (Left) detected with ACSM5 antibody)
Mouse ACSM5 Monoclonal Antibody | anti-ACSM5 antibody
ACSM5 antibody
Applications
Immunofluorescence, Western Blot
Purity
ACSM5 antibody was purified by affinity chromatography.
Synonyms
ACSM5; Monoclonal Antibody; ACSM5 antibody; Monoclonal ACSM5; Anti-ACSM5; FLJ20581; ACSM 5; ACSM-5; anti-ACSM5 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
4B11
Purity/Purification
ACSM5 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
0.5-1 mg/ml (varies by lot)
Sequence Length
579
Applicable Applications for anti-ACSM5 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 1:100
WB: 1:2000
WB: 1:2000
Biological Significance
ACSM5 has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids.
Cross-Reactivity
Human
Immunogen
ACSM5 antibody was raised in Mouse using full length human recombinant protein of human ACSM5 produced in HEK293T cell as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACSM5 protein (Right) or empty vector (Left) detected with ACSM5 antibody)
Western Blot (WB)
(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant ACSM5 protein (Right) or empty vector (Left) detected with ACSM5 antibody)
Related Product Information for anti-ACSM5 antibody
Mouse monoclonal ACSM5 antibody
Product Categories/Family for anti-ACSM5 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,320 Da
NCBI Official Full Name
acyl-coenzyme A synthetase ACSM5, mitochondrial
NCBI Official Synonym Full Names
acyl-CoA synthetase medium-chain family member 5
NCBI Official Symbol
ACSM5
NCBI Protein Information
acyl-coenzyme A synthetase ACSM5, mitochondrial
UniProt Protein Name
Acyl-coenzyme A synthetase ACSM5, mitochondrial
Protein Family
UniProt Gene Name
ACSM5
UniProt Synonym Gene Names
MACS3
UniProt Entry Name
ACSM5_HUMAN
Uniprot Description
ACSM5: Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4- unsaturated acids (in vitro). Belongs to the ATP-dependent AMP-binding enzyme family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 6.2.1.2; Carbohydrate Metabolism - butanoate; Mitochondrial; Ligase
Chromosomal Location of Human Ortholog: 16p12.3
Cellular Component: mitochondrial matrix
Molecular Function: butyrate-CoA ligase activity; GTP binding; metal ion binding; ATP binding
Biological Process: fatty acid metabolic process
Research Articles on ACSM5
Similar Products
Product Notes
The ACSM5 acsm5 (Catalog #AAA834384) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ACSM5 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). IF: 1:100 WB: 1:2000. Researchers should empirically determine the suitability of the ACSM5 acsm5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACSM5, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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