Western Blot (WB)
((DANRE) eif3eb Antibody (Center) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the (DANRE) eif3eb antibody detected the (DANRE) eif3eb protein (arrow).)
Rabbit EIF3E Polyclonal Antibody | anti-EIF3E antibody
EIF3E Antibody (Center)
Gene Names
EIF3E; INT6; EIF3S6; EIF3-P48; eIF3-p46
Reactivity
Human, Zebrafish (Predicted Reactivity: Xenopus, Bovine, Chicken, Monkey, Mouse, Rat)
Applications
Western Blot, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
EIF3E; Polyclonal Antibody; EIF3E Antibody (Center); Eukaryotic translation initiation factor 3 subunit E {ECO:0000255|HAMAP-Rule:MF_03004}; eIF3e {ECO:0000255|HAMAP-Rule:MF_03004}; Eukaryotic translation initiation factor 3 subunit 6 {ECO:0000255|HAMAP-Rule:MF_03004}; Viral integration site protein INT-6 homolog; eIF-3 p48 {ECO:0000255|HAMAP-Rule:MF_03004}; EIF3E {ECO:0000255|HAMAP-Rule:MF_03004}; anti-EIF3E antibody
Host
Rabbit
Reactivity
Human, Zebrafish (Predicted Reactivity: Xenopus, Bovine, Chicken, Monkey, Mouse, Rat)
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This EIF3E antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-276 amino acids from the Central region of human EIF3E.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
248-276
Sequence Length
445
Applicable Applications for anti-EIF3E antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
((DANRE) eif3eb Antibody (Center) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the (DANRE) eif3eb antibody detected the (DANRE) eif3eb protein (arrow).)
Western Blot (WB)
((DANRE) eif3eb Antibody (Center) western blot analysis in zebra fish brain tissue lysates (35ug/lane).This demonstrates the (DANRE) eif3eb antibody detected the (DANRE) eif3eb protein (arrow).)
Western Blot (WB)
(EIF3E Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3E antibody detected the EIF3E protein (arrow).)
Western Blot (WB)
(EIF3E Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the EIF3E antibody detected the EIF3E protein (arrow).)
Related Product Information for anti-EIF3E antibody
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of posttermination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. Required for nonsense-mediated mRNA decay (NMD); may act in conjunction with UPF2 to divert mRNAs from translation to the NMD pathway. May interact with MCM7 and EPAS1 and regulate the proteasome-mediated degradation of these proteins.
Product Categories/Family for anti-EIF3E antibody
References
Grzmil, M., et al. Oncogene 29(28):4080-4089(2010)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
Morris, C., et al. EMBO Rep. 8(6):596-602(2007)
Sirchia, R., et al. Biol. Chem. 388(5):457-465(2007)
Zhou, M., et al. Proc. Natl. Acad. Sci. U.S.A. 105(47):18139-18144(2008)
Masutani, M., et al. EMBO J. 26(14):3373-3383(2007)
Morris, C., et al. EMBO Rep. 8(6):596-602(2007)
Sirchia, R., et al. Biol. Chem. 388(5):457-465(2007)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52221
NCBI Official Full Name
eukaryotic translation initiation factor 3 subunit E
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 3, subunit E
NCBI Official Symbol
EIF3E
NCBI Official Synonym Symbols
INT6; EIF3S6; EIF3-P48; eIF3-p46
NCBI Protein Information
eukaryotic translation initiation factor 3 subunit E
UniProt Protein Name
Eukaryotic translation initiation factor 3 subunit E
UniProt Gene Name
EIF3E
UniProt Synonym Gene Names
eIF3e
UniProt Entry Name
EIF3E_HUMAN
Uniprot Description
EIF3E: eukaryotic translation initiation factor 3 subunit 2. Binds to the 40S ribosome and promotes the binding of methionyl-tRNAi and mRNA. eIF-3 is composed of at least 12 different subunits. Phosphorylated by TGF-beta type II receptor.
Protein type: Translation initiation; Translation
Chromosomal Location of Human Ortholog: 8q22-q23
Cellular Component: nucleoplasm; eukaryotic translation initiation factor 3 complex; PML body; membrane; cytoplasm; nucleus; chromatin; cytosol
Molecular Function: protein binding; translation initiation factor activity; protein N-terminus binding
Biological Process: negative regulation of translational initiation; cellular protein metabolic process; translation; mRNA catabolic process, nonsense-mediated decay; translational initiation; gene expression; formation of translation preinitiation complex; regulation of translational initiation
Research Articles on EIF3E
Similar Products
Product Notes
The EIF3E eif3e (Catalog #AAA9214205) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 248-276. The EIF3E Antibody (Center) reacts with Human, Zebrafish (Predicted Reactivity: Xenopus, Bovine, Chicken, Monkey, Mouse, Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's EIF3E can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the EIF3E eif3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EIF3E, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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