Rabbit MAPT Polyclonal Antibody | anti-MAPT antibody

MAPT, Phosphorylated (T205) (Tau Protein, Microtubule-associated Protein Tau, DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND) (AP)

Cat. Num. AAA6426012

• Gene Names: MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: MAPT; Polyclonal Antibody; Phosphorylated (T205) (Tau Protein; Microtubule-associated Protein Tau; DDPAC; FLJ31424; FTDP-17; MAPTL; MGC138549; MSTD; MTBT1; MTBT2; PPND) (AP); anti-MAPT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MAPT, phosphorylated T205. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MAPT antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding T205 of human MAPT.
• Conjugate: AP
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from mouse brain tissue, using phospho-MAPT-T205 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer, using phospho-MAPT-T205 antibody.)

Product Categories/Family for anti-MAPT antibody

Antibodies; Microtubule-associated Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 294862258
• NCBI GeneID: 4137
• NCBI Accession #: NP_001116538.2
• NCBI GenBank Nucleotide #: NM_001123066.3
• UniProt Accession #: P10636; P18518; Q14799; Q15549; Q15550; Q15551; Q1RMF6; Q53YB1; Q5CZI7; Q5XWF0; Q6QT54; Q9UDJ3
• Molecular Weight: 78,928 Da
• NCBI Official Full Name: microtubule-associated protein tau isoform 6
• NCBI Official Synonym Full Names: microtubule-associated protein tau
• NCBI Official Symbol: MAPT
• NCBI Official Synonym Symbols: TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17
• NCBI Protein Information: microtubule-associated protein tau; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; microtubule-associated protein tau, isoform 4; G protein beta1/gamma2 subunit-interacting factor 1
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAPT
• UniProt Synonym Gene Names: MAPTL; MTBT1; TAU; PHF-tau
• UniProt Entry Name: TAU_HUMAN

NCBI Description

This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq, Jul 2008]

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.1

Cellular Component: microtubule; microtubule associated complex; growth cone; axon; tubulin complex; plasma membrane; axoneme; cytosol

Molecular Function: protein binding; enzyme binding; structural constituent of cytoskeleton; microtubule binding; apolipoprotein binding; protein kinase binding; SH3 domain binding

Biological Process: axon extension; apoptosis; positive regulation of microtubule polymerization; positive regulation of axon extension; axon cargo transport; neuron migration; microtubule cytoskeleton organization and biogenesis; adult walking behavior; regulation of microtubule polymerization; mitochondrion transport along microtubule; negative regulation of intracellular transport; generation of neurons; regulation of autophagy; cell structure disassembly during apoptosis

Disease: Supranuclear Palsy, Progressive, 1; Pick Disease Of Brain; Frontotemporal Dementia; Parkinson-dementia Syndrome; Parkinson Disease, Late-onset; Frontotemporal Lobar Degeneration With Tdp43 Inclusions, Grn-related