Rabbit anti-Human CPT1B Polyclonal Antibody | anti-CPT1B antibody

CPT1B, CT (CPT1B, KIAA1670, Carnitine O-palmitoyltransferase 1, muscle isoform, Carnitine O-palmitoyltransferase I, muscle isoform, Carnitine palmitoyltransferase 1B, Carnitine palmitoyltransferase I-like protein) (FITC)

Cat. Num. AAA6288486

• Gene Names: CPT1B; CPTI; CPT1M; MCPT1; CPT1-M; CPTI-M; M-CPT1; MCCPT1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CPT1B; Polyclonal Antibody; CT (CPT1B; KIAA1670; Carnitine O-palmitoyltransferase 1; muscle isoform; Carnitine O-palmitoyltransferase I; Carnitine palmitoyltransferase 1B; Carnitine palmitoyltransferase I-like protein) (FITC); Carnitine palmitoyltransferase I-like protein; anti-CPT1B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CPT1B antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CPT1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 743-771 amino acids from the C-terminal region of human CPT1B.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CPT1B Antibody (C-term) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the CPT1B antibody detected the CPT1B protein (arrow).)

Related Product Information for anti-CPT1B antibody

The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene.

Product Categories/Family for anti-CPT1B antibody

Antibodies; KIAA; orf

References

Matsuo, T., et al. Biochem. Genet. 48 (3-4), 193-201 (2010): Miljkovic, I., et al. Obesity (Silver Spring) 17(7):1396-1401(2009). Miyagawa, T., et al. PLoS ONE 4 (4), E5394 (2009): van der Leij, F.R., et al. J. Biol. Chem. 277(30):26994-27005(2002). Yamazaki, N., et al. J. Biol. Chem. 275(41):31739-31746(2000).

NCBI and Uniprot Product Information

• NCBI GI #: 223468678
• NCBI GeneID: 1375
• NCBI Accession #: NP_001138609.1; NP_001138607.1
• NCBI GenBank Nucleotide #: NM_001145137.1
• UniProt Accession #: Q92523; Q13389; Q99655; Q9BY90; B7Z4U4; B7Z5T8; E9PCP2
• Molecular Weight: 87,801 Da
• NCBI Official Full Name: carnitine O-palmitoyltransferase 1, muscle isoform isoform a
• NCBI Official Synonym Full Names: carnitine palmitoyltransferase 1B (muscle)
• NCBI Official Symbol: CPT1B
• NCBI Official Synonym Symbols: CPTI; CPT1M; MCPT1; CPT1-M; CPTI-M; M-CPT1; MCCPT1
• NCBI Protein Information: carnitine O-palmitoyltransferase 1, muscle isoform; carnitine palmitoyltransferase I-like protein; carnitine O-palmitoyltransferase I, mitochondrial muscle isoform
• UniProt Protein Name: Carnitine O-palmitoyltransferase 1, muscle isoform
• Protein Family: Carnitine O-palmitoyltransferase
• UniProt Gene Name: CPT1B
• UniProt Synonym Gene Names: KIAA1670; CPT1-M; CPT I; CPTI-M
• UniProt Entry Name: CPT1B_HUMAN

NCBI Description

The protein encoded by this gene, a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

CPT1B: a member of the carnitine/choline acetyltransferase family, is the rate-controlling enzyme of the long-chain fatty acid beta-oxidation pathway in muscle mitochondria. This enzyme is required for the net transport of long-chain fatty acyl-CoAs from the cytoplasm into the mitochondria. Multiple transcript variants encoding different isoforms have been found for this gene, and read-through transcripts are expressed from the upstream locus that include exons from this gene. [provided by RefSeq, Jun 2009]

Protein type: Lipid Metabolism - fatty acid; Membrane protein, multi-pass; Membrane protein, integral; Transferase; EC 2.3.1.21

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: mitochondrial outer membrane; mitochondrion; integral to membrane

Molecular Function: carnitine O-palmitoyltransferase activity

Biological Process: fatty acid beta-oxidation; cellular lipid metabolic process; carnitine shuttle