Rabbit MAPKAPK2 Polyclonal Antibody | anti-MAPKAPK2 antibody

Rabbit anti MAPKAPK2 (pThr334)

Cat. Num. AAA225282

• Gene Names: MAPKAPK2; MK2; MK-2; MAPKAP-K2
• Reactivity: Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified whole serum by affinity chromatography.
• Synonyms: MAPKAPK2; Polyclonal Antibody; Rabbit anti MAPKAPK2 (pThr334); MAPKAPK2 (pThr334) Antibody; anti-MAPKAPK2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified whole serum by affinity chromatography.
• Form/Format: Liquid in phosphate buffered saline with 0.02% sodium azide, 50% glycerol.
• Sequence Length: 400

• Applicable Applications for anti-MAPKAPK2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-Paraffin: 1:50-1:100; WB: 1:500-1:2000
• Immunogen: Phospho specific-peptide corresponding to residues surrounding threonine 334 of human MAPKAPK2
• Preperation: Antiserum to MAPKAPK2 (pThr334) was raised by repeated immunization of rabbits with highly purified antigen.
• Preparation and Storage: Store at -20 degree C.; Storage in frost-free freezers is not recommended. This product should be stored undiluted.; Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch

Western Blot (WB)

(Western blot analysis of extracts from HeLa cells untreated or treated with UV (for 15-30 minutes) using Rabbit anti MAPKAPK2 (pThr334) antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti MAPKAPK2 (pThr334) antibody)

Related Product Information for anti-MAPKAPK2 antibody

Rabbit anti MAPKAPK2 (pThr334) antibody recognizes MAP kinase-activated protein kinase 2 (MAPKAPK2), when phosphorylated at threonine 334. MAPKAPK2 belongs to the CAMK serine/threonine protein kinase family and is activated in response to cellular stress and cytokines. MAPKAPK2 is phosphorylated at threonine 222, serine 272 and threonine 334 by MAPK14 (Ben-Levy et al. 1995).

NCBI and Uniprot Product Information

• NCBI GI #: 1346538
• NCBI GeneID: 9261
• UniProt Accession #: P49137; Q5SY30; Q5SY41; Q8IYD6
• Molecular Weight: 42,203 Da
• NCBI Official Full Name: MAP kinase-activated protein kinase 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase-activated protein kinase 2
• NCBI Official Symbol: MAPKAPK2
• NCBI Official Synonym Symbols: MK2; MK-2; MAPKAP-K2
• NCBI Protein Information: MAP kinase-activated protein kinase 2
• UniProt Protein Name: MAP kinase-activated protein kinase 2
• UniProt Gene Name: MAPKAPK2
• UniProt Synonym Gene Names: MAPK-activated protein kinase 2; MAPKAP kinase 2; MAPKAP-K2; MAPKAPK-2; MK-2; MK2

NCBI Description

This gene encodes a member of the Ser/Thr protein kinase family. This kinase is regulated through direct phosphorylation by p38 MAP kinase. In conjunction with p38 MAP kinase, this kinase is known to be involved in many cellular processes including stress and inflammatory responses, nuclear export, gene expression regulation and cell proliferation. Heat shock protein HSP27 was shown to be one of the substrates of this kinase in vivo. Two transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Stress-activated serine/threonine-protein kinase involved in cytokine production, endocytosis, reorganization of the cytoskeleton, cell migration, cell cycle control, chromatin remodeling, DNA damage response and transcriptional regulation. Following stress, it is phosphorylated and activated by MAP kinase p38-alpha/MAPK14, leading to phosphorylation of substrates. Phosphorylates serine in the peptide sequence, Hyd-X-R-X2-S, where Hyd is a large hydrophobic residue. Phosphorylates ALOX5, CDC25B, CDC25C, CEP131, ELAVL1, HNRNPA0, HSP27/HSPB1, KRT18, KRT20, LIMK1, LSP1, PABPC1, PARN, PDE4A, RCSD1, RPS6KA3, TAB3 and TTP/ZFP36. Phosphorylates HSF1; leading to the interaction with HSP90 proteins and inhibiting HSF1 homotrimerization, DNA-binding and transactivation activities (PubMed:16278218). Mediates phosphorylation of HSP27/HSPB1 in response to stress, leading to the dissociation of HSP27/HSPB1 from large small heat-shock protein (sHsps) oligomers and impairment of their chaperone activities and ability to protect against oxidative stress effectively. Involved in inflammatory response by regulating tumor necrosis factor (TNF) and IL6 production post-transcriptionally: acts by phosphorylating AU-rich elements (AREs)-binding proteins ELAVL1, HNRNPA0, PABPC1 and TTP/ZFP36, leading to the regulation of the stability and translation of TNF and IL6 mRNAs. Phosphorylation of TTP/ZFP36, a major post-transcriptional regulator of TNF, promotes its binding to 14-3-3 proteins and reduces its ARE mRNA affinity, leading to inhibition of dependent degradation of ARE-containing transcripts. Phosphorylates CEP131 in response to cellular stress induced by ultraviolet irradiation which promotes binding of CEP131 to 14-3-3 proteins and inhibits formation of novel centriolar satellites (PubMed:26616734). Also involved in late G2/M checkpoint following DNA damage through a process of post-transcriptional mRNA stabilization: following DNA damage, relocalizes from nucleus to cytoplasm and phosphorylates HNRNPA0 and PARN, leading to stabilization of GADD45A mRNA. Involved in toll-like receptor signaling pathway (TLR) in dendritic cells: required for acute TLR-induced macropinocytosis by phosphorylating and activating RPS6KA3.