Human Ras-related protein Rab-14 ELISA Kit | RAB14 elisa kit

Human Ras-related protein Rab-14 ELISA Kit

Cat. Num. AAA761681

• Gene Names: RAB14; FBP; RAB-14
• Reactivity: Human
• Synonyms: Ras-related protein Rab-14; Human Ras-related protein Rab-14 ELISA Kit; RAB14/Ras-related protein Rab-14/FBP/RAB-14; RAB14 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of RAB14. No significant cross-reactivity or interference between RAB14 and analogues was observed.
• Sequence Length: 215

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.781-50ng/ml
• Sensitivity: 0.469ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RAB14 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 19923483
• NCBI GeneID: 51552
• NCBI Accession #: NP_057406.2
• NCBI GenBank Nucleotide #: NM_016322.3
• Molecular Weight: 23,897 Da
• NCBI Official Full Name: ras-related protein Rab-14
• NCBI Official Synonym Full Names: RAB14, member RAS oncogene family
• NCBI Official Symbol: RAB14
• NCBI Official Synonym Symbols: FBP; RAB-14
• NCBI Protein Information: ras-related protein Rab-14
• UniProt Protein Name: Ras-related protein Rab-14
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB14
• UniProt Entry Name: RAB14_HUMAN

NCBI Description

RAB14 belongs to the large RAB family of low molecular mass GTPases that are involved in intracellular membrane trafficking. These proteins act as molecular switches that flip between an inactive GDP-bound state and an active GTP-bound state in which they recruit downstream effector proteins onto membranes (Junutula et al., 2004 [PubMed 15004230]).[supplied by OMIM, Mar 2009]

Uniprot Description

RAB14: a protein of the GTPase superfamily, Rab family. May be involved in vesicular trafficking and neurotransmitter release. Localized to the inner face of the cell membrane by a lipid-anchor.

Protein type: G protein, monomeric; G protein; G protein, monomeric, Rab

Chromosomal Location of Human Ortholog: 9q33.2

Cellular Component: apical plasma membrane; cytoplasmic vesicle membrane; cytosol; early endosome; early endosome membrane; Golgi membrane; Golgi stack; intracellular; intracellular membrane-bound organelle; late endosome; lysosomal membrane; lysosome; nuclear envelope-endoplasmic reticulum network; perinuclear region of cytoplasm; phagocytic vesicle; plasma membrane; recycling endosome; rough endoplasmic reticulum; trans-Golgi network; trans-Golgi network transport vesicle

Molecular Function: GDP binding; glycoprotein binding; GTP binding; GTPase activity; myosin V binding; protein binding

Biological Process: apical protein localization; body fluid secretion; defense response to bacterium; embryonic development; endocytic recycling; fibroblast growth factor receptor signaling pathway; Golgi to endosome transport; intracellular transport; metabolic process; protein transport; regulation of protein localization; small GTPase mediated signal transduction; vesicle-mediated transport