Rabbit MAPK9/MAPK10 Polyclonal Antibody | anti-MAPK9/MAPK10 antibody

MAPK9/MAPK10, Phosphorylated (T183) (Mitogen-activated Protein Kinase 9, MAP Kinase 9, Mitogen-activated Protein Kinase 10, MAP Kinase 10, JNK2) (FITC)

Cat. Num. AAA6425850

• Gene Names: MAPK9; JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: MAPK9/MAPK10; Polyclonal Antibody; Phosphorylated (T183) (Mitogen-activated Protein Kinase 9; MAP Kinase 9; Mitogen-activated Protein Kinase 10; MAP Kinase 10; JNK2) (FITC); anti-MAPK9/MAPK10 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MAPK9/MAPK10, phosphorylated T183. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Concentration: ~1mg/ml (varies by lot)
• Sequence Length: 424

• Applicable Applications for anti-MAPK9/MAPK10 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; WB: 1:500-1:1000; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding T183 of human MAPK9/MAPK10.
• Conjugate: FITC
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells using phospho-MAPK9/MAPK10-T183 antibody and the same antibody preincubated with blocking peptide.)

Product Categories/Family for anti-MAPK9/MAPK10 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 85700366
• NCBI GeneID: 5601
• UniProt Accession #: P45984/P53779; P45984
• Molecular Weight: 4654
• NCBI Official Full Name: Mitogen-activated protein kinase 9
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 9
• NCBI Official Symbol: MAPK9
• NCBI Official Synonym Symbols: JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA
• NCBI Protein Information: mitogen-activated protein kinase 9

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase targets specific transcription factors, and thus mediates immediate-early gene expression in response to various cell stimuli. It is most closely related to MAPK8, both of which are involved in UV radiation induced apoptosis, thought to be related to the cytochrome c-mediated cell death pathway. This gene and MAPK8 are also known as c-Jun N-terminal kinases. This kinase blocks the ubiquitination of tumor suppressor p53, and thus it increases the stability of p53 in nonstressed cells. Studies of this gene's mouse counterpart suggest a key role in T-cell differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Sep 2008]