Western Blot (WB)
(Western Blot analysis of NG2 in HeLa whole cell lysate using MBS602463.)
Mouse anti-Human MCSP Monoclonal Antibody | anti-CSPG4 antibody
MCSP (Melanoma-associated Chondroitin Sulfate Proteoglycan, Chondroitin Sulfate Proteoglycan 4 (Melanoma Associated), Chondroitin Sulfate Proteoglycan 4, Chondroitin Sulfate Proteoglycan NG2, CSPG4, MCSPG, MEL-CSPG, Melanoma Chondroitin Sulfate Proteoglyc
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: Flow Cytometry: 1:10-1:1000
Western Blot: 1:1000
Immunofluorescence (IC): 1:100
Immunoprecipitation: 1:10-1:100
Immunohistochemistry(Paraffin/frozen): 1:200
Western Blot (WB)
(Western Blot analysis of NG2 in HeLa whole cell lysate using MBS602463.)
Western Blot (WB)
(Western Blot analysis of NG2 in HeLa whole cell lysate using MBS602463.)
Immunohistochemistry (IHC)
(Immunohistochemistry-paraffin staining of NG2 in human brain using DAB with hematoxylin counterstain with MBS602463.)
Immunohistochemistry (IHC)
(Immunohistochemistry-paraffin staining of NG2 in human brain using DAB with hematoxylin counterstain with MBS602463.)
Flow Cytometry (FC/FACS)
(Flow Cytometry of MBS602463 (1:400) in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).)
Flow Cytometry (FC/FACS)
(Flow Cytometry of MBS602463 (1:400) in MCF7 cells using an Alexa Fluor 488 secondary (shown in green) alongside unstained cells (shown in red).)
NCBI and Uniprot Product Information
NCBI Description
A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. [provided by RefSeq]
Uniprot Description
NG2: a transmembrane chondrotin sulfate proteoglycan expressed on several types of immature progenitor cells and human malignant melanoma cells. Plays a role in cell proliferation and motility of endothelial cells during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. May regulate MPP16-dependent degradation and invasion of type I collagen participating to melanoma cells invasion properties. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through Cdc42, ACK1 and P130Cas. May activate FAK and ERK signaling cascades. PKC-alpha-mediated NG2 phosphorylation may be a key step for initiating cell polarization and motility.
Protein type: Membrane protein, integral; Cell adhesion
Chromosomal Location of Human Ortholog: 15q24.2
Cellular Component: lysosomal lumen; focal adhesion; cell surface; Golgi lumen; integral to plasma membrane; apical plasma membrane; extracellular region
Molecular Function: signal transducer activity; protein kinase binding
Biological Process: chondroitin sulfate metabolic process; cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; chondroitin sulfate biosynthetic process; glycosaminoglycan metabolic process; activation of MAPK activity; carbohydrate metabolic process; chondroitin sulfate catabolic process; tissue remodeling; pathogenesis; angiogenesis; glial cell migration; dermatan sulfate biosynthetic process; transmembrane receptor protein tyrosine kinase signaling pathway
Research Articles on CSPG4
2. Human skin aging is associated with reduced expression of the stem cell markers beta1 integrin and MCSP
3. NG2 expression on culture-expanded mesenchymal stromal cells was investigated by flow cytometry.
4. NG2 was present in the evolving astroglial scar after human traumatic spinal cord injury, and, therefore, might play an important role in the blockade of successful CNS regeneration.
5. Co-expression and interaction of NG2 and galectin-3 in human glioma cells establish a molecular basis for the NG2/galectin-3 interaction.
6. Observational study of gene-disease association. (HuGE Navigator)
7. NG2 has a role in metastasis formation in soft-tissue sarcoma patients
8. the coexpression of KOR-SA35443 (kappa opioid receptor) and NG2 (chondroitin sulfate proteoglycan) in result of karyotype abnormal changes may predict a poor prognosis in Pro-B acute lymphoblastic leukemia
9. A Lm-based vaccine against HMW-MAA can trigger cell-mediated immune responses to this antigen that can target not only tumor cells but also pericytes in the tumor vasculature.
10. High NG2/MPG expression correlated with multidrug resistance mediated by increased activation of alpha3beta1 integrin/PI3K signaling and their downstream targets, promoting cell survival
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Product Notes
The CSPG4 cspg4 (Catalog #AAA602463) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MCSP (Melanoma-associated Chondroitin Sulfate Proteoglycan, Chondroitin Sulfate Proteoglycan 4 (Melanoma Associated), Chondroitin Sulfate Proteoglycan 4, Chondroitin Sulfate Proteoglycan NG2, CSPG4, MCSPG, MEL-CSPG, Melanoma Chondroitin Sulfate Proteoglyc reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MCSP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Dilution: Flow Cytometry: 1:10-1:1000 Western Blot: 1:1000 Immunofluorescence (IC): 1:100 Immunoprecipitation: 1:10-1:100 Immunohistochemistry(Paraffin/frozen): 1:200. Researchers should empirically determine the suitability of the CSPG4 cspg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCSP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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