Rat Amphiregulin ELISA Kit | AR elisa kit

Rat Amphiregulin ELISA Kit

Cat. Num. AAA763740

• Gene Names: AREG; AR; SDGF; AREGB; CRDGF
• Reactivity: Rat
• Synonyms: Amphiregulin; Rat Amphiregulin ELISA Kit; AREG/amphiregulin/AR/AREG/AREGB/Colorectum cell-derived growth factor/CRDGF/schwannoma-derived growth factor/SDGF; AR elisa kit

• Reactivity: Rat
• Sequence Length: 252

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for AR elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Rat AR antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat AR antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat AR amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofRat AR can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502199
• NCBI GeneID: 374
• NCBI Accession #: NP_001648.1
• NCBI GenBank Nucleotide #: NM_001657.3
• Molecular Weight: 27,895 Da
• NCBI Official Full Name: amphiregulin preproprotein
• NCBI Official Synonym Full Names: amphiregulin
• NCBI Official Symbol: AREG
• NCBI Official Synonym Symbols: AR; SDGF; AREGB; CRDGF
• NCBI Protein Information: amphiregulin
• UniProt Protein Name: Amphiregulin
• Protein Family: Allatostatin
• UniProt Gene Name: AREG
• UniProt Synonym Gene Names: CRDGF
• UniProt Entry Name: AREG_HUMAN

NCBI Description

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]

Uniprot Description

AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cell surface; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; extracellular space; Golgi membrane; integral to membrane; nucleus

Molecular Function: cytokine activity; epidermal growth factor receptor binding; growth factor activity; protein binding

Biological Process: cell proliferation; cell-cell signaling; cellular protein metabolic process; COPII coating of Golgi vesicle; epidermal growth factor receptor signaling pathway; ER to Golgi vesicle-mediated transport; G-protein coupled receptor protein signaling pathway; glial cell proliferation; negative regulation of osteoblast differentiation; neurite development; positive regulation of cell proliferation; positive regulation of DNA replication; positive regulation of phosphorylation; post-translational protein modification; protein amino acid N-linked glycosylation via asparagine; response to cAMP; response to estradiol stimulus; response to glucocorticoid stimulus; response to hydrogen peroxide; response to peptide hormone stimulus