Rabbit MAPK11 Polyclonal Antibody | anti-MAPK11 antibody

Anti-MAPK11 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100829

• Gene Names: MAPK11; P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: MAPK11; Polyclonal Antibody; Anti-MAPK11 Antibody; Rabbit Polyclonal; MAPK11 Antibody; Rabbit PAb; Antigen Affinity Purified; mitogen-activated protein kinase 11; Anti-p38-2 Antibody; Anti-P38B Antibody; Anti-p38Beta Antibody; Anti-P38BETA2 Antibody; Anti-PRKM11 Antibody; Anti-SAPK2 Antibody; Anti-SAPK2B Antibody; anti-MAPK11 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human MAPK11
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-MAPK11 antibody: Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:2000; IHC-P: 1:500-1:2000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the N-terminus of the Human MAPK11
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human MAPK11, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-MAPK11 rabbit polyclonal antibody at 1:500 dilution Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:41 kDa Observed band size:36 kDa)

Immunohistochemistry (IHC)

(Immunochemical staining of human MAPK11 in human breast carcinoma with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human MAPK11 in human liver with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of MAPK11 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-HUMAN MAPK11 polyclonal antibody (dilution ratio: 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

Related Product Information for anti-MAPK11 antibody

The p38 kinases are one of the four subgroups of the mitogen-activated protein kinase (MAPK) superfamily which are involved in innate immunity. The p38 subfamily includes four members namely p38alpha (MAPK14), p38beta (MAPK11), p38gamma (MAPK12), and p38delta (MAPK13), regulates the activation of several transcription factors. MAPK11 (Mitogen-Activated Protein Kinase 11) is a Protein Coding gene. This gene encodes a member of a family of protein kinases that are involved in the integration of biochemical signals for a wide variety of cellular processes, including cell proliferation, differentiation, transcriptional regulation, and development. MAPK11 is widely expressed in fat, the brain, and other tissues. The encoded protein can be activated by proinflammatory cytokines and environmental stresses through phosphorylation by mitogen-activated protein kinase kinases (MKKs).

Product Categories/Family for anti-MAPK11 antibody

VEGF Signaling Pathway

NCBI and Uniprot Product Information

• NCBI GI #: 134047835
• NCBI GeneID: 5600
• UniProt Accession #: Q15759; O00284; O15472; Q2XNF2; A8K730; B0LPG1; B7Z630; E7ETQ1; L7RT27
• Molecular Weight: 364
• NCBI Official Full Name: Mitogen-activated protein kinase 11
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 11
• NCBI Official Symbol: MAPK11
• NCBI Official Synonym Symbols: P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2
• NCBI Protein Information: mitogen-activated protein kinase 11; MAP kinase 11; MAP kinase p38 beta; stress-activated protein kinase-2; stress-activated protein kinase-2b; mitogen-activated protein kinase p38-2; mitogen-activated protein kinase p38 beta
• UniProt Protein Name: Mitogen-activated protein kinase 11
• UniProt Gene Name: MAPK11
• UniProt Synonym Gene Names: PRKM11; SAPK2; SAPK2B; MAP kinase 11; MAPK 11; MAP kinase p38 beta; p38b; SAPK2b
• UniProt Entry Name: MK11_HUMAN

NCBI Description

This gene encodes a member of a family of protein kinases that are involved in the integration of biochemical signals for a wide variety of cellular processes, including cell proliferation, differentiation, transcriptional regulation, and development. The encoded protein can be activated by proinflammatory cytokines and environmental stresses through phosphorylation by mitogen activated protein kinase kinases (MKKs). Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]

Uniprot Description

P38B: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Inhibited by SB203580 and pyridinyl-imidazole related compounds.

Protein type: Protein kinase, CMGC; EC 2.7.11.24; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; p38 subfamily; MAPK/p38 subfamily

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: nucleoplasm; cytosol

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: mitochondrion organization and biogenesis; nerve growth factor receptor signaling pathway; transcription, DNA-dependent; activation of MAPK activity; organelle organization and biogenesis; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; toll-like receptor 10 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; toll-like receptor 5 signaling pathway; muscle cell differentiation; regulation of transcription factor activity; Ras protein signal transduction; toll-like receptor signaling pathway; response to stress; positive regulation of muscle cell differentiation; innate immune response; gene expression; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway; toll-like receptor 4 signaling pathway