Rabbit MAP4K4 Polyclonal Antibody | anti-MAP4K4 antibody

MAP4K4 Rabbit Polyclonal

Cat. Num. AAA7601216

• Gene Names: MAP4K4; HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Synonyms: MAP4K4; Polyclonal Antibody; MAP4K4 Rabbit Polyclonal; anti-MAP4K4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 1165

• Applicable Applications for anti-MAP4K4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen: Mitogen-activated protein kinase kinase kinase kinase 4
• Preparation and Storage: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20 degree C for 24 months (Avoid repeated freeze / thaw cycles.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human skeletal muscle using MBS7601216 (MAP4K4 antibody) at dilution of 1:50)

Western Blot (WB)

(human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with MBS7601216 (MAP4K4 antibody) at dilution of 1:1000)

NCBI and Uniprot Product Information

• NCBI GI #: 336020353
• NCBI GeneID: 9448
• NCBI Accession #: NP_004825.3
• NCBI GenBank Nucleotide #: NM_004834.4
• Molecular Weight: 141,929 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 4 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 4
• NCBI Official Symbol: MAP4K4
• NCBI Official Synonym Symbols: HGK; NIK; MEKKK4; FLH21957; HEL-S-31
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 4
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 4
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K4
• UniProt Synonym Gene Names: HGK; KIAA0687; NIK; MEK kinase kinase 4; MEKKK 4
• UniProt Entry Name: M4K4_HUMAN

NCBI Description

The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

HGK: a Ser/Thr kinase of the STE20 subfamily. May play a role in the response to environmental stress and cytokines such as TNF-alpha. May interact with the SH3 domain of the adapter proteins Nck. Binds, via its CNH regulatory domain, to the N- terminal region of SPG3A. Upregulated in primary tumors and tumor cell lines. Required for anchorage-independent growth and ras-dependent focus formation in multiple cell lines. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; MSN subfamily

Chromosomal Location of Human Ortholog: 2q11.2-q12

Cellular Component: cytoplasm

Molecular Function: ATP binding; creatine kinase activity; protein binding; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: microvillus biogenesis; negative regulation of apoptosis; protein amino acid phosphorylation; regulation of JNK cascade