Rabbit Map4k3 Polyclonal Antibody | anti-Map4k3 antibody

Mouse Map4k3 Antibody (Center)

Cat. Num. AAA9212248

• Gene Names: Map4k3; Glk; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1; 4833416M01Rik; 4833416M07Rik; 9530052P13Rik
• Reactivity: Human, mouse (Predicted Reactivity: Rat)
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: Map4k3; Polyclonal Antibody; Mouse Map4k3 Antibody (Center); Mitogen-activated protein kinase kinase kinase kinase 3; Germinal center kinase-related protein kinase; GLK; MAPK/ERK kinase kinase kinase 3; MEK kinase kinase 3; MEKKK 3; anti-Map4k3 antibody

• Host: Rabbit
• Reactivity: Human, mouse (Predicted Reactivity: Rat)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This Mouse Map4k3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 501-529 amino acids from the Central region of mouse Map4k3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 501-529
• Sequence Length: 894

• Applicable Applications for anti-Map4k3 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: MOUSE
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Mouse Map4k3 Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the Map4k3 antibody detected the Map4k3 protein (arrow).)

Western Blot (WB)

(Mouse Map4k3 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Map4k3 antibody detected the Map4k3 protein (arrow).)

Flow Cytometry (FC/FACS)

(Mouse Map4k3 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Mouse Map4k3 Antibody (Center) (MBS9212248)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map4k3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Map4k3 antibody

Map4k3 may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).

Product Categories/Family for anti-Map4k3 antibody

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 594140610
• NCBI GeneID: 225028
• NCBI Accession #: NP_001277274.1
• NCBI GenBank Nucleotide #: NM_001290345.1
• UniProt Accession #: Q99JP0; Q9CUS8; E9PWI4
• Molecular Weight: 101119
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 3
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 3
• NCBI Official Symbol: Map4k3
• NCBI Official Synonym Symbols: Glk; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1; 4833416M01Rik; 4833416M07Rik; 9530052P13Rik
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 3
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 3
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: Map4k3
• UniProt Synonym Gene Names: GLK; MEK kinase kinase 3; MEKKK 3
• UniProt Entry Name: M4K3_MOUSE

Uniprot Description

KHS2: an ubiquitous protein kinase of the STE20 family. May play a role in the response to environmental stress. Regulated by amino acids and acts upstream of mTORC1 and JNK. Required for maximal S6K and 4E-BP1 phosphorylation. Interacts with PPP2R5E, a regulatory subunit of PP2A. Following amino acid withdrawal, pS170 is dephosphorylated via PP2A. The dephosphorylation of pS170 by PP2A in response to amino acid restriction inhibits mTORC1 signaling. The inhibition of PPP2R5E expression prevents the dephosphorylation of KHS2 pS170, impairing mTORC1 inhibition during amino acid withdrawal. Three alternatively spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; STE group; STE20 family; KHS subfamily

Cellular Component: cytoplasm; intracellular

Molecular Function: transferase activity; protein serine/threonine kinase activity; protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: response to stress; protein amino acid phosphorylation; phosphorylation; response to UV