Western Blot (WB)
(Anti-PDX1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
Rabbit anti-Human PDX1 Polyclonal Antibody | anti-PDX1 antibody
Anti-PDX1 Antibody, Rabbit Polyclonal
IP: 0.2-1uL/mg of lysate
Western Blot (WB)
(Anti-PDX1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-PDX1 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: Caco-2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:30 kDaObserved band size:38 kDa(We are unsure as to the identity of these extra bands.))
Immunoprecipitation (IP)
(PDX1 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-PDX1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PDX1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)
Immunoprecipitation (IP)
(PDX1 was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate0.5 uL anti-PDX1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PDX1 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 38 kDaObserved band size: 38 kDa)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a transcriptional activator of several genes, including insulin, somatostatin, glucokinase, islet amyloid polypeptide, and glucose transporter type 2. The encoded nuclear protein is involved in the early development of the pancreas and plays a major role in glucose-dependent regulation of insulin gene expression. Defects in this gene are a cause of pancreatic agenesis, which can lead to early-onset insulin-dependent diabetes mellitus (NIDDM), as well as maturity onset diabetes of the young type 4 (MODY4). [provided by RefSeq, Jul 2008]
Uniprot Description
PDX1: Activates insulin, somatostatin, glucokinase, islet amyloid polypeptide and glucose transporter type 2 gene transcription. Particularly involved in glucose-dependent regulation of insulin gene transcription. Binds preferentially the DNA motif 5'-[CT]TAAT[TG]-3'. During development, specifies the early pancreatic epithelium, permitting its proliferation, branching and subsequent differentiation. At adult stage, required for maintaining the hormone-producing phenotype of the beta-cell. Interacts with the basic helix-loop-helix domains of TCF3(E47) and NEUROD1 and with HMG-I(Y). Interacts with SPOP. Interacts with the methyltransferase SETD7. Duodenum and pancreas (Langerhans islet beta cells and small subsets of endocrine non-beta-cells, at low levels in acinar cells). Belongs to the Antp homeobox family. IPF1/XlHbox-8 subfamily.
Protein type: DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 13q12.1
Cellular Component: nucleus; cytosol
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein heterodimerization activity; protein complex binding; chromatin binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; response to nicotine; central nervous system development; response to glucocorticoid stimulus; exocrine pancreas development; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; response to chlorate; response to vitamin; negative regulation of cell proliferation; detection of glucose; morphogenesis of embryonic epithelium; positive regulation of cell proliferation; response to wounding; response to iron(II) ion; positive regulation of DNA binding; response to drug; organ regeneration; smoothened signaling pathway; generation of precursor metabolites and energy; organ senescence; positive regulation of insulin secretion; glucose metabolic process; stem cell differentiation; liver development; endocrine pancreas development; organ morphogenesis; response to leucine; gut development; insulin secretion; response to cytokine stimulus; positive regulation of transcription from RNA polymerase II promoter; nitric oxide mediated signal transduction
Disease: Maturity-onset Diabetes Of The Young, Type 4; Diabetes Mellitus, Permanent Neonatal; Pancreatic Agenesis 1; Diabetes Mellitus, Noninsulin-dependent
Research Articles on PDX1
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Product Notes
The PDX1 pdx1 (Catalog #AAA8100336) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-PDX1 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PDX1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:1000 IP: 0.2-1uL/mg of lysate. Researchers should empirically determine the suitability of the PDX1 pdx1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDX1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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