Rabbit anti-Human, Mouse Map4k3 Polyclonal Antibody | anti-MAP4K3 antibody

Map4k3, ID (Map4k3, Mitogen-activated protein kinase kinase kinase kinase 3, Germinal center kinase-related protein kinase, MAPK/ERK kinase kinase kinase 3)

Cat. Num. AAA6004302

• Gene Names: MAP4K3; GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Map4k3; Polyclonal Antibody; ID (Map4k3; Mitogen-activated protein kinase kinase kinase kinase 3; Germinal center kinase-related protein kinase; MAPK/ERK kinase kinase kinase 3); Anti -Map4k3; anti-MAP4K3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-MAP4K3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50; Immunohistochemistry: 1:10-50
• Immunogen: Mouse Map4k3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 500-529 amino acids from the Central region of Mouse Map4k3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in HepG2 cell line lysates (35ug/lane) using MBS6004302. This demonstrates that MBS6004302 detected the Map4k3 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse Neuro-2a cell line lysates (35ug/lane) using MBS6004302. This demonstrates that MBS6004302 detected the Map4k3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded mouse heart tissue using MBS6004302 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6004302 for immunohistochemistry.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Neuro-2a cells using MBS6004302 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-MAP4K3 antibody

Map4k3 may play a role in the response to environmental stress. Appears to act upstream of the JUN N-terminal pathway (By similarity).

Product Categories/Family for anti-MAP4K3 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 394025669
• NCBI GeneID: 8491
• NCBI Accession #: NP_001257354.1
• NCBI GenBank Nucleotide #: NM_001270425.1
• UniProt Accession #: Q8IVH8; Q6IQ39; Q8IVH7; Q9UDM5; Q9Y6R5
• Molecular Weight: 101,316 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase kinase 3 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase kinase 3
• NCBI Official Symbol: MAP4K3
• NCBI Official Synonym Symbols: GLK; MEKKK3; MEKKK 3; MAPKKKK3; RAB8IPL1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase kinase 3; MEK kinase kinase 3; MAPK/ERK kinase kinase kinase 3; germinal center kinase-like kinase; germinal center kinase-related protein kinase
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase kinase 3
• Protein Family: Mitogen-activated protein kinase kinase kinase kinase
• UniProt Gene Name: MAP4K3
• UniProt Synonym Gene Names: RAB8IPL1; GLK; MEK kinase kinase 3; MEKKK 3
• UniProt Entry Name: M4K3_HUMAN

NCBI Description

This gene encodes a member of the mitogen-activated protein kinase kinase kinase kinase family. The encoded protein activates key effectors in cell signalling, among them c-Jun. Alternatively spliced transcripts encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]

Uniprot Description

KHS2: an ubiquitous protein kinase of the STE20 family. May play a role in the response to environmental stress. Regulated by amino acids and acts upstream of mTORC1 and JNK. Required for maximal S6K and 4E-BP1 phosphorylation. Interacts with PPP2R5E, a regulatory subunit of PP2A. Following amino acid withdrawal, pS170 is dephosphorylated via PP2A. The dephosphorylation of pS170 by PP2A in response to amino acid restriction inhibits mTORC1 signaling. The inhibition of PPP2R5E expression prevents the dephosphorylation of KHS2 pS170, impairing mTORC1 inhibition during amino acid withdrawal. Three alternatively spliced isoforms have been described.

Protein type: Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, STE; STE group; STE20 family; KHS subfamily

Chromosomal Location of Human Ortholog: 2p22.1

Cellular Component: cytoplasm

Molecular Function: protein serine/threonine kinase activity; protein binding; ATP binding; receptor signaling protein serine/threonine kinase activity; protein kinase activity

Biological Process: regulation of catalytic activity; JNK cascade; protein amino acid phosphorylation; response to UV