UNC5A recombinant protein

UNC5A Protein, Human, Recombinant (ECD, His Tag)

Cat. Num. AAA8121406

• Gene Names: UNC5A; UNC5H1
• Purity: >95% as determined by SDS-PAGE
• Synonyms: UNC5A; UNC5A Protein; Human; Recombinant (ECD; His Tag); Human UNC5A/UNC5H1 Protein (ECD; UNC5H1 Protein; unc-5 homolog A (C. elegans); UNC5A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Tyr306

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Gln 26
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human UNC5A (NP_588610.2)(Met1-Tyr306) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for UNC5A recombinant protein

Background: The netrin-1 receptor Uncoordinated Phenotype-5A, or UNC5A, plays an important role in predicting response to DNA damage induced by chemotherapeutic drug and regulating cell death in bladder cancer. Moreover, UNC5A is cumulatively downregulated by the unfolding protein response (UPR) at the transcriptional level in vitro and at the translational level both in vitro and in vivo. Also, UNC5A is a novel transcriptional target of p53 and plays a role in p53-dependent apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 62243567
• NCBI GeneID: 90249
• NCBI Accession #: NP_588610.2
• NCBI GenBank Nucleotide #: NM_133369.2
• UniProt Accession #: Q6ZN44; Q8TF26; Q96GP4; B2RXE6
• Molecular Weight: 92,932 Da
• NCBI Official Full Name: netrin receptor UNC5A
• NCBI Official Synonym Full Names: unc-5 homolog A (C. elegans)
• NCBI Official Symbol: UNC5A
• NCBI Official Synonym Symbols: UNC5H1
• NCBI Protein Information: netrin receptor UNC5A; unc-5 homolog 1; netrin receptor Unc5h1; protein unc-5 homolog 1; protein unc-5 homolog A; unc5 (C.elegans homolog) a
• UniProt Protein Name: Netrin receptor UNC5A
• Protein Family: Netrin receptor
• UniProt Gene Name: UNC5A
• UniProt Synonym Gene Names: KIAA1976; UNC5H1
• UniProt Entry Name: UNC5A_HUMAN

Uniprot Description

UNC5A: Receptor for netrin required for axon guidance. Mediates axon repulsion of neuronal growth cones in the developing nervous system upon ligand binding. Axon repulsion in growth cones may be caused by its association with DCC that may trigger signaling for repulsion. It also acts as a dependence receptor required for apoptosis induction when not associated with netrin ligand. Belongs to the unc-5 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cell development/differentiation; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: integral to membrane; intrinsic to plasma membrane; lipid raft; plasma membrane

Biological Process: anterior/posterior axon guidance; apoptosis; axon guidance; neurite development; programmed cell death