Rabbit UBASH3A Polyclonal Antibody | anti-UBASH3A antibody

UBASH3A Polyclonal Antibody

Cat. Num. AAA3006276

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: UBASH3A; Polyclonal Antibody; UBASH3A Polyclonal Antibody; Ubiquitin-associated and SH3 domain-containing protein A; Cbl-interacting protein 4; CLIP4; Suppressor of T-cell receptor signaling 2; STS-2; T-cell ubiquitin ligand 1; TULA-1; TULA1; STS2; anti-UBASH3A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: UBASH3A polyclonal antibody detects endogenous levels of UBASH3A protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 623

• Applicable Applications for anti-UBASH3A antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: WB: 1:500-1:1000; ICC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 356-401 of Human UBASH3A.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of UBASH3A polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:sp2/0 cell lysateLane3:H9C2 cell lysate)

Related Product Information for anti-UBASH3A antibody

TULA (T cell ubiquitin ligand), also known as UBASH3A (ubiquitin associated and SH3 domain containing A), STS-2 or CLIP4, is a 661 amino acid protein that localizes to both the nucleus and the cytoplasm and contains one SH3 domain and one UBA domain. Expressed at high levels in thymus, bone marrow, spleen and peripheral blood leukocytes, TULA exists as either a homodimer or a homo-oligomer that interferes with the degradation of receptor-type tyrosine kinases and promotes the accumulation of activated receptors on the cell surface. Additionally, TULA is part of an EGFR- and Cbl-containing complex that interacts with ubiquitinated proteins. The gene encoding TULA, which maps to human chromosome 21, may be involved in the pathogenesis of type I diabetes. Multiple isoforms of TULA are produced due to alternative splicing events.

NCBI and Uniprot Product Information

• NCBI GI #: 49640014
• NCBI Accession #: NP_001001895.1
• NCBI GenBank Nucleotide #: NP_001001895.1
• UniProt Accession #: P57075
• Molecular Weight: ~ 74kDa
• NCBI Official Full Name: ubiquitin-associated and SH3 domain-containing protein A isoform 2
• UniProt Protein Name: Ubiquitin-associated and SH3 domain-containing protein A
• Protein Family: Ubiquitin-associated and SH3 domain-containing protein
• UniProt Gene Name: UBASH3A
• UniProt Synonym Gene Names: STS2; CLIP4; STS-2; TULA-1
• UniProt Entry Name: UBS3A_HUMAN

Uniprot Description

STS2: Interferes with CBL-mediated down-regulation and degradation of receptor-type tyrosine kinases. Promotes accumulation of activated target receptors, such as T-cell receptors, EGFR and PDGFRB, on the cell surface. Exhibits negligigle protein tyrosine phosphatase activity at neutral pH. May act as a dominant-negative regulator of UBASH3B-dependent dephosphorylation. May inhibit dynamin-dependent endocytic pathways by functionally sequestering dynamin via its SH3 domain. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleus; cytosol

Biological Process: negative regulation of T cell receptor signaling pathway; regulation of cytokine production