Rabbit anti-Human, Mouse Map3k7 Polyclonal Antibody | anti-Map3k7 antibody

Map3k7, NT (Map3k7, Tak1, Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1) (PE)

Cat. Num. AAA6318670

• Gene Names: Map3k7; Tak1; C87327; B430101B05
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Map3k7; Polyclonal Antibody; NT (Map3k7; Tak1; Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1) (PE); Transforming growth factor-beta-activated kinase 1; anti-Map3k7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-Map3k7 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Mouse Map3k7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-160 amino acids from the N-terminal region of Mouse Map3k7.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Mouse Map3k7 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Map3k7 antibody detected the Map3k7 protein (arrow).)

Western Blot (WB)

(Western blot analysis of Map3k7 (arrow) using rabbit polyclonal Mouse Map3k7 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k7 gene.)

Western Blot (WB)

(Mouse Map3k7 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Map3k7 antibody detected the Map3k7 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Map3k7 Antibody (N-term) (AP13919a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Map3k7 antibody

Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.

Product Categories/Family for anti-Map3k7 antibody

Antibodies; Protein Kinases

References

Kajino-Sakamoto, R., et al. J. Immunol. 185(8):4729-4737(2010). Wu, Z.H., et al. Mol. Cell 40(1):75-86(2010). Scholz, R., et al. J. Biol. Chem. 285(33):25753-25766(2010). Greenblatt, M.B., et al. J. Clin. Invest. 120(7):2457-2473(2010). Bettermann, K., et al. Cancer Cell 17(5):481-496(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 27881429
• NCBI GeneID: 26409
• NCBI Accession #: NP_766276.1
• NCBI GenBank Nucleotide #: NM_172688.3
• UniProt Accession #: O43318; Q62073
• Molecular Weight: 64,228 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 7 isoform A
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase kinase 7
• NCBI Official Symbol: Map3k7
• NCBI Official Synonym Symbols: Tak1; C87327; B430101B05
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 7; TGF-beta activated kinase 1; TGF-beta-activated kinase 1; mitogen activated protein kinase kinase kinase 7; transforming growth factor beta-activated kinase 1; transforming growth factor-beta-activated kin
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 7
• Protein Family: MAP3K7 C-terminal-like protein
• UniProt Gene Name: MAP3K7
• UniProt Synonym Gene Names: TAK1; TGF-beta-activated kinase 1
• UniProt Entry Name: M3K7_HUMAN

Uniprot Description

TAK1: a protein kinase of the MLK family. Mediates signal transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. Also activates MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced isoforms have been reported. Three splice variant isoforms have been described.

Protein type: EC 2.7.11.25; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group; MLK family; TAK1 subfamily

Chromosomal Location of Human Ortholog: 6q15

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; endosome membrane; IkappaB kinase complex; cytosol; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of JNK activity; I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; activation of MAPK activity; apoptosis; stress-activated MAPK cascade; positive regulation of T cell cytokine production; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; transforming growth factor beta receptor signaling pathway; JNK cascade; angiogenesis; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; activation of NF-kappaB-inducing kinase; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interleukin-2 production; toll-like receptor signaling pathway; innate immune response; positive regulation of T cell activation; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; neural tube formation