Rabbit anti-Human, Mouse Map3k7 Polyclonal Antibody | anti-Map3k7 antibody

Map3k7, NT (Map3k7, Tak1, Mitogen-activated protein kinase kinase kinase 7, Transforming growth factor-beta-activated kinase 1)

Cat. Num. AAA6005920

• Gene Names: Map3k7; Tak1
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Map3k7; Polyclonal Antibody; NT (Map3k7; Tak1; Mitogen-activated protein kinase kinase kinase 7; Transforming growth factor-beta-activated kinase 1); Anti -Map3k7; anti-Map3k7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Map3k7 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: Mouse Map3k7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-160 amino acids from the N-terminal region of Mouse Map3k7.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Map3k7 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the Map3k7 antibody detected the Map3k7 protein (arrow).)

Western Blot (WB)

(Western blot analysis of Map3k7 (arrow) using rabbit polyclonal Mouse Map3k7 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the Map3k7 gene.)

Western Blot (WB)

(Mouse Map3k7 Antibody (N-term) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the Map3k7 antibody detected the Map3k7 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Map3k7 Antibody (N-term) (AP13919a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Map3k7 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-Map3k7 antibody

Component of a protein kinase signal transduction cascade. Mediator of TRAF6 and TGF-beta signal transduction. Activates IKBKB and MAPK8 in response to TRAF6 signaling. Stimulates NF-kappa-B activation and the p38 MAPK pathway. In osmotic stress signaling, plays a major role in the activation of MAPK8/JNK, but not that of NF-kappa-B.

Product Categories/Family for anti-Map3k7 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 281485586
• NCBI GeneID: 313121
• NCBI Accession #: NP_001101390.2
• NCBI GenBank Nucleotide #: NM_001107920.2
• UniProt Accession #: P0C8E4
• Molecular Weight: 67,200 Da
• NCBI Official Full Name: mitogen-activated protein kinase kinase kinase 7
• NCBI Official Synonym Full Names: mitogen activated protein kinase kinase kinase 7
• NCBI Official Symbol: Map3k7
• NCBI Official Synonym Symbols: Tak1
• NCBI Protein Information: mitogen-activated protein kinase kinase kinase 7; TGF-beta activated kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase kinase 7
• Protein Family: MAP3K7 C-terminal-like protein
• UniProt Gene Name: Map3k7
• UniProt Entry Name: M3K7_RAT

Uniprot Description

TAK1: a protein kinase of the MLK family. Mediates signal transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. Also activates MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced isoforms have been reported. Three splice variant isoforms have been described.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC 2.7.11.25; TKL group; MLK family; TAK1 subfamily

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; IkappaB kinase complex; intracellular; nucleus

Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activity; protein binding; MAP kinase kinase kinase activity; magnesium ion binding; ATP binding; protein kinase activity

Biological Process: positive regulation of JNK activity; positive regulation of I-kappaB kinase/NF-kappaB cascade; activation of MAPKK activity; transcription, DNA-dependent; activation of MAPK activity; apoptosis; stress-activated MAPK cascade; MAPKKK cascade; activation of NF-kappaB-inducing kinase; positive regulation of T cell cytokine production; positive regulation of JNK cascade; protein amino acid phosphorylation; regulation of transcription, DNA-dependent; transforming growth factor beta receptor signaling pathway; positive regulation of interleukin-2 production; JNK cascade; angiogenesis; neural tube formation; I-kappaB phosphorylation