Western Blot (WB)
(Western blot analysis of MAP3K12 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Rabbit MAP3K12 Polyclonal Antibody | anti-MAP3K12 antibody
MAP3K12 Antibody
Gene Names
MAP3K12; DLK; MUK; ZPK; ZPKP1; MEKK12
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
MAP3K12; Polyclonal Antibody; MAP3K12 Antibody; Dlk; Dual leucine zipper bearing kinase; Dual leucine zipper kinase DLK; Leucine-zipper protein kinase; M3K12_HUMAN; Map3k12; MAPK-upstream kinase; MEKK12; Mitogen-activated protein kinase kinase kinase 12; Mixed lineage kinase; MUK; Protein kinase MUK; ZPK; ZPKP1; anti-MAP3K12 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
MAP3K12 antibody detects endogenous levels of total MAP3K12
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
892
Applicable Applications for anti-MAP3K12 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide
Subcellular Location
Cytoplasm. Membrane.
Tissue Specificity
Highly expressed in brain and kidney.
Predicted Cross Reactivity
Pig, Bovine, Horse, Sheep, Rabbit, Dog
Similarity
Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of MAP3K12 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of MAP3K12 expression in HEK293 cells, The lane on the left is treated with the antigen-specific peptide.)
Related Product Information for anti-MAP3K12 antibody
Description: May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
Subunit Structure: Interacts with MBIP.
Post-translational Modifications: Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated (By similarity).
Similarity: Interacts with MBIP through the leucine-zipper motif. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Subunit Structure: Interacts with MBIP.
Post-translational Modifications: Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated (By similarity).
Similarity: Interacts with MBIP through the leucine-zipper motif. Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 95 kDa
Predicted: 94 kDa
Predicted: 94 kDa
NCBI Official Full Name
mitogen-activated protein kinase kinase kinase 12 isoform 1
NCBI Official Synonym Full Names
mitogen-activated protein kinase kinase kinase 12
NCBI Official Symbol
MAP3K12
NCBI Official Synonym Symbols
DLK; MUK; ZPK; ZPKP1; MEKK12
NCBI Protein Information
mitogen-activated protein kinase kinase kinase 12
UniProt Protein Name
Mitogen-activated protein kinase kinase kinase 12
Protein Family
UniProt Gene Name
MAP3K12
UniProt Synonym Gene Names
ZPK; DLK; ZPK; MUK
NCBI Description
This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010]
Uniprot Description
May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
Research Articles on MAP3K12
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Product Notes
The MAP3K12 map3k12 (Catalog #AAA9603865) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAP3K12 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAP3K12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the MAP3K12 map3k12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP3K12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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