Western Blot (WB)
(Western blot analysis of MAP2K2-S222 in Y79 cell line lysates (35ug/lane). MAP2K2 (arrow) was detected using the purified Pab.)
Rabbit MAP2K2 Polyclonal Antibody | anti-MAP2K2 antibody
MAP2K2 Antibody (S222)
Western Blot (WB)
(Western blot analysis of MAP2K2-S222 in Y79 cell line lysates (35ug/lane). MAP2K2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of MAP2K2-S222 in Y79 cell line lysates (35ug/lane). MAP2K2 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of MAP2K2 (arrow) using rabbit polyclonal MAP2K2-S222. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2).)
Western Blot (WB)
(Western blot analysis of MAP2K2 (arrow) using rabbit polyclonal MAP2K2-S222. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAP2K2 gene (Lane 2).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with MAP2K2 Antibody (S222) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Tran, H., et al., Mol. Cell. Biol. 23(20):7177-7188 (2003).
Li, S.P., et al., Cancer Res. 63(13):3473-3477 (2003).
Li, Y., et al., J. Biol. Chem. 278(16):13663-13671 (2003).
Liu, X., et al., J. Biol. Chem. 277(42):39312-39319 (2002).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. Mutations in this gene cause cardiofaciocutaneous syndrome (CFC syndrome), a disease characterized by heart defects, mental retardation, and distinctive facial features similar to those found in Noonan syndrome. The inhibition or degradation of this kinase is also found to be involved in the pathogenesis of Yersinia and anthrax. A pseudogene, which is located on chromosome 7, has been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
MEK2: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf and Mos kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK2 and ERK3. A component of MAP kinase signal transduction pathways involved in mitogen growth factor signal transduction.
Protein type: Protein kinase, dual-specificity (non-receptor); Protein kinase, STE; EC 2.7.12.2; Kinase, protein; STE group; STE7 family
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: peroxisomal membrane; Golgi apparatus; microtubule; internal side of plasma membrane; focal adhesion; mitochondrion; endoplasmic reticulum; early endosome; extracellular region; cell cortex; intercellular junction; cytosol; perinuclear region of cytoplasm; late endosome; cytoplasm; nucleus
Molecular Function: MAP kinase kinase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein binding; protein-tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; protein complex scaffold; ATP binding; PDZ domain binding
Biological Process: axon guidance; peptidyl-tyrosine phosphorylation; activation of MAPKK activity; nerve growth factor receptor signaling pathway; activation of MAPK activity; stress-activated MAPK cascade; pathogenesis; toll-like receptor 3 signaling pathway; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; small GTPase mediated signal transduction; toll-like receptor 4 signaling pathway; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; MAPKKK cascade; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of stress-activated MAPK cascade; Ras protein signal transduction; toll-like receptor signaling pathway; insulin receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway
Disease: Cardiofaciocutaneous Syndrome 4
Research Articles on MAP2K2
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Product Notes
The MAP2K2 map2k2 (Catalog #AAA9214905) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 200-229. The MAP2K2 Antibody (S222) reacts with Human (Predicted Reactivity: Drosophila, Hamster, Mouse, Rabbit, Rat, Xenopus, Chicken) and may cross-react with other species as described in the data sheet. AAA Biotech's MAP2K2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC). WB~~1:1000. Researchers should empirically determine the suitability of the MAP2K2 map2k2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAP2K2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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