Rabbit MAP2 Polyclonal Antibody | anti-MAP2 antibody

MAP2 (Microtubule-associated Protein 2, DKFZp686I2148, MAP2A, MAP2B, MAP2C) (HRP)

Cat. Num. AAA6425323

• Gene Names: MAP2; MAP2A; MAP2B; MAP2C
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by affinity chromatography.
• Synonyms: MAP2; Polyclonal Antibody; MAP2 (Microtubule-associated Protein 2; DKFZp686I2148; MAP2A; MAP2B; MAP2C) (HRP); anti-MAP2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes MAP2. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-MAP2 antibody: Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IHC: 1:50-1:200; WB: 1:500-1:2000; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human MAP2.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using MAP2 antibody.)

Product Categories/Family for anti-MAP2 antibody

Antibodies; Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 87578396
• NCBI GeneID: 4133
• NCBI Accession #: NP_002365.3
• NCBI GenBank Nucleotide #: NM_002374.3
• UniProt Accession #: P11137; Q17S04; Q8IUX2; Q99975; Q99976
• Molecular Weight: 199,526 Da
• NCBI Official Full Name: microtubule-associated protein 2 isoform 1
• NCBI Official Synonym Full Names: microtubule-associated protein 2
• NCBI Official Symbol: MAP2
• NCBI Official Synonym Symbols: MAP2A; MAP2B; MAP2C
• NCBI Protein Information: microtubule-associated protein 2; MAP-2
• UniProt Protein Name: Microtubule-associated protein 2
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAP2
• UniProt Synonym Gene Names: MAP-2
• UniProt Entry Name: MTAP2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the microtubule-associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron-specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2010]

Uniprot Description

MAP2: a member of the microtubule-associated protein (MAP) family. Its exact function is unknown but MAPs appear to stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. Expressed at high levels in neural tissue and the prostate. Four alternatively spliced transcript variants have been reported.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q34-q35

Cellular Component: microtubule; microtubule associated complex; cell soma; cytoplasm; nucleolus; dendritic shaft

Molecular Function: calmodulin binding; protein binding; microtubule binding; structural molecule activity

Biological Process: axonogenesis; dendrite morphogenesis; microtubule bundle formation; neurite development; central nervous system neuron development