Human H1 Histone Family, Member 0 (H1F0) ELISA Kit | H1F0 elisa kit

Human H1 Histone Family, Member 0 (H1F0) ELISA Kit

Cat. Num. AAA2024953

• Gene Names: H1F0; H10; H1FV
• Reactivity: Human
• Synonyms: H1 Histone Family; Member 0 (H1F0); Human H1 Histone Family; Member 0 (H1F0) ELISA Kit; H1FV; H10; H1F0 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of H1 Histone Family, Member 0 (H1F0).; No significant cross-reactivity or interference between H1 Histone Family, Member 0 (H1F0) and analogues was observed.
• Sequence Length: 194

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.78-50ng/mL
• Sensitivity: < 0.28ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level H1 Histone Family, Member 0 (H1F0) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level H1 Histone Family, Member 0 (H1F0) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for H1F0 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to H1 Histone Family, Member 0 (H1F0). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to H1 Histone Family, Member 0 (H1F0). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain H1 Histone Family, Member 0 (H1F0), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of H1 Histone Family, Member 0 (H1F0) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for H1F0 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 119580592
• NCBI GeneID: 3005
• Molecular Weight: 19,167 Da
• NCBI Official Full Name: H1 histone family, member 0
• NCBI Official Synonym Full Names: H1 histone family, member 0
• NCBI Official Symbol: H1F0
• NCBI Official Synonym Symbols: H10; H1FV
• NCBI Protein Information: histone H1.0
• UniProt Protein Name: Histone H1.0
• Protein Family: Histone
• UniProt Gene Name: H1F0
• UniProt Synonym Gene Names: H1FV
• UniProt Entry Name: H10_HUMAN

NCBI Description

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H1 family. [provided by RefSeq, Jul 2008]

Uniprot Description

H1F0: Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. Belongs to the histone H1/H5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: nucleoplasm; Golgi apparatus; nuclear chromatin; nucleosome; nucleus; actin cytoskeleton

Molecular Function: protein binding; chromatin DNA binding

Biological Process: nucleosome assembly; apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis