Rabbit anti-Human, Mouse MAP Kinase 4 Polyclonal Antibody | anti-MAPK4 antibody

MAP Kinase 4, CT (Mitogen-activated Protein Kinase 4, MAPK 4, MAPK4, Extracellular Signal-regulated Kinase 4, ERK4, ERK-4, MAP Kinase Isoform p63, p63-MAPK, p63MAPK, PRKM4) (Biotin)

Cat. Num. AAA6486728

• Gene Names: MAPK4; ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAP Kinase 4; Polyclonal Antibody; CT (Mitogen-activated Protein Kinase 4; MAPK 4; MAPK4; Extracellular Signal-regulated Kinase 4; ERK4; ERK-4; MAP Kinase Isoform p63; p63-MAPK; p63MAPK; PRKM4) (Biotin); EC=2.7.11.24; anti-MAPK4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ERK4. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MAPK4 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-1:50; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human ERK4 (KLH).
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-ERK4 Pab in mouse brain tissue lysate. ERK4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with ERK4 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-MAPK4 antibody

Antibodies; Protein Kinases

References

1. Robinson, M.J., et al., Curr. Opin. Cell Biol. 9(2):180-186 (1997). 2. Davis, R.J., Mol. Reprod. Dev. 42(4):459-467 (1995). 3. Seger, R., et al., FASEB J. 9(9):726-735 (1995). 4. Li, L., et al., Oncogene 9(2):647-649 (1994). 5. Zhu, A.X., et al., Mol. Cell. Biol. 14(12):8202-8211 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 89276769
• NCBI GeneID: 5596
• NCBI Accession #: NP_002738.2
• NCBI GenBank Nucleotide #: NM_002747.3
• UniProt Accession #: P31152; Q0VG04; A1A4C4
• Molecular Weight: 65,922 Da
• NCBI Official Full Name: mitogen-activated protein kinase 4 isoform 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 4
• NCBI Official Symbol: MAPK4
• NCBI Official Synonym Symbols: ERK4; ERK-4; PRKM4; p63MAPK; p63-MAPK
• NCBI Protein Information: mitogen-activated protein kinase 4
• UniProt Protein Name: Mitogen-activated protein kinase 4
• UniProt Gene Name: MAPK4
• UniProt Synonym Gene Names: ERK4; PRKM4; MAP kinase 4; MAPK 4; ERK-4; p63-MAPK
• UniProt Entry Name: MK04_HUMAN

NCBI Description

Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

ERK4: a protein kinase of the MAPK family. Phosphorylation and activations leads to translocation into the nucleus where it phosphorylates nuclear targets.

Protein type: Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; Kinase, protein; EC 2.7.11.24; CMGC group; MAPK family; MAPK/ERK subfamily; ERK subfamily

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: ATP binding; MAP kinase activity; protein binding; protein heterodimerization activity; protein homodimerization activity; protein kinase binding; protein serine/threonine kinase activity

Biological Process: cell cycle; MAPKKK cascade; protein amino acid phosphorylation