Rabbit anti-Human SPAG5 Polyclonal Antibody | anti-SPAG5 antibody

SPAG5 Polyclonal Antibody

Cat. Num. AAA3007041

• Reactivity: Human
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: SPAG5; Polyclonal Antibody; SPAG5 Polyclonal Antibody; Sperm-associated antigen 5; Astrin; Deepest; Mitotic spindle-associated protein p126; MAP126; anti-SPAG5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SPAG5 polyclonal antibody detects endogenous levels of SPAG5 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1193

• Applicable Applications for anti-SPAG5 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human SPAG5
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of SPAG5 pAb at 1:500 dilutionLane1:Myla2059 whole cell lysate (40ug) Lane2:HEK293T whole cell lysate (40ug) Lane3:HuT78 whole cell lysate (40ug))

Related Product Information for anti-SPAG5 antibody

SPAG5 (sperm associated antigen 5), also known as MAP126, hMAP126, DEEPEST or Astrin, is a 1, 193 amino acid protein that is expressed at high levels in testis and at lower levels in liver, placenta, pancreas, colon and thymus. Localized specifically to spindle poles during prophase and throughout the spindle during metaphase and anaphase, SPAG5 is essential for proper spindle formation and is thought to be involved in the dynamic and functional regulation of mitotic spindles. Additionally, SPAG5 plays a role in sister chromatid cohesion and subsequent chromatid separation and, via its association with chromatids, is an important regulator of chromosome integrity. SPAG5 has a long stalk and a globular head domain through which if forms a homodimer that is characterized by aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.

NCBI and Uniprot Product Information

• NCBI GI #: 73623035
• NCBI Accession #: NP_006452.3
• NCBI GenBank Nucleotide #: NP_006452.3
• UniProt Accession #: Q96R06
• Molecular Weight: ~ 134, 150kDa
• NCBI Official Full Name: sperm-associated antigen 5
• UniProt Protein Name: Sperm-associated antigen 5
• Protein Family: Sperm-associated antigen
• UniProt Gene Name: SPAG5
• UniProt Synonym Gene Names: MAP126
• UniProt Entry Name: SPAG5_HUMAN

Uniprot Description

Function: Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments. Ref.1 Ref.6 Ref.15

Subunit structure: Homodimer, with a globular head domain and a long stalk. Homooligomer; the globular head domains associate, resulting in aster-like structures. Binds to microtubules in the mitotic spindle. Interacts with DCLRE1B/Apollo. Part of an astrin (SPAG5)-kinastrin (SKAP) complex containing KNSTRN, SPAG5, PLK1, DYNLL1 and SGOL2. Interacts with KNSTRN. Ref.1 Ref.6 Ref.11 Ref.15

Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › spindle pole. Chromosome › centromere › kinetochore. Note: In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends

By similarity. Ref.1 Ref.2 Ref.6 Ref.15

Tissue specificity: Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon. Ref.2

Sequence caution: The sequence AAD02813.1 differs from that shown. Reason: Frameshift at position 1093. The sequence AAL06396.2 differs from that shown. Reason: Frameshift at position 1135.