Western Blot (WB)
(Western Blot: Sample: Recombinant MAN1A1, Human.)
Rabbit anti-Human Mannosidase Alpha Class 1A Member 1 Polyclonal Antibody | anti-MAN1A1 antibody
Polyclonal Antibody to Mannosidase Alpha Class 1A Member 1 (MAN1A1)
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Recombinant MAN1A1, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant MAN1A1, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded kidney tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a class I mammalian Golgi 1,2-mannosidase which is a type II transmembrane protein. This protein catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides and belongs to family 47 of glycosyl hydrolases. [provided by RefSeq, Jul 2012]
Uniprot Description
MAN1A1: Involved in the maturation of Asn-linked oligosaccharides. Progressively trim alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2). Belongs to the glycosyl hydrolase 47 family.
Protein type: Calcium-binding; EC 3.2.1.113; Endoplasmic reticulum; Glycan Metabolism - N-glycan biosynthesis; Hydrolase; Membrane protein, integral
Chromosomal Location of Human Ortholog: 6q22.31
Cellular Component: cytosol; endoplasmic reticulum; endoplasmic reticulum-Golgi intermediate compartment; Golgi apparatus; Golgi membrane; integral component of membrane; membrane
Molecular Function: calcium ion binding; mannosidase activity; mannosyl-oligosaccharide 1,2-alpha-mannosidase activity
Biological Process: N-glycan processing; protein glycosylation
Research Articles on MAN1A1
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Product Notes
The MAN1A1 man1a1 (Catalog #AAA2034058) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mannosidase Alpha Class 1A Member 1 (MAN1A1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Mannosidase Alpha Class 1A Member 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the MAN1A1 man1a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Mannosidase Alpha Class 1A Member 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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