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Western Blot (WB) (Western Blot: Sample: Mouse Heart lysate; Primary Ab: 4ug/ml Rabbit Anti-Human ACTC1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Mouse anti-Human Actin Alpha 1 (ACTC1) Monoclonal Antibody | anti-ACTC1 antibody

Monoclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1)

Gene Names
ACTC1; ACTC; ASD5; CMD1R; CMH11; LVNC4
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Protein A + Protein G affinity chromatography
Synonyms
Actin Alpha 1 (ACTC1); Monoclonal Antibody; Monoclonal Antibody to Actin Alpha 1; Cardiac Muscle (ACTC1); ACTC; CMD1R; CMD1-R; ACT-a1; anti-ACTC1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b Kappa
Clone Number
C7
Specificity
The antibody is a mouse monoclonal antibody raised against ACTC1. It has been selected for its ability to recognize ACTC1 in immunohistochemical staining and western blotting.
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Liquid
Concentration
1mg/mL (varies by lot)
Sequence Length
377
Applicable Applications for anti-ACTC1 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
Western Blot: 0.5-5ug/mL
Immunocytochemistry in formalin fixed cells: 5-30ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-30ug/mL
Immunohistochemistry in paraffin section: 5-30ug/mL
Immunogen
Recombinant Actin Alpha 1, Cardiac Muscle (ACTC1)
Cross Reactivity
Human, Mouse
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2054089
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Mouse Heart lysate; Primary Ab: 4ug/ml Rabbit Anti-Human ACTC1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Heart lysate; Primary Ab: 4ug/ml Rabbit Anti-Human ACTC1 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant ACTC1, Human.)

Western Blot (WB) (Western Blot: Sample: Recombinant ACTC1, Human.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
70
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
42,019 Da
NCBI Official Full Name
actin, alpha cardiac muscle 1
NCBI Official Synonym Full Names
actin, alpha, cardiac muscle 1
NCBI Official Symbol
ACTC1
NCBI Official Synonym Symbols
ACTC; ASD5; CMD1R; CMH11; LVNC4
NCBI Protein Information
actin, alpha cardiac muscle 1
UniProt Protein Name
Actin, alpha cardiac muscle 1
Protein Family
UniProt Gene Name
ACTC1
UniProt Synonym Gene Names
ACTC

NCBI Description

Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in this gene have been associated with idiopathic dilated cardiomyopathy (IDC) and familial hypertrophic cardiomyopathy (FHC). [provided by RefSeq, Jul 2008]

Uniprot Description

ACTC1: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Cell development/differentiation; Contractile; Cytoskeletal; Motility/polarity/chemotaxis; Myosin-binding

Chromosomal Location of Human Ortholog: 15q14

Cellular Component: actin filament; cytoplasm; cytosol; extracellular space; filopodium; focal adhesion; I band; lamellipodium; membrane; sarcomere

Molecular Function: ATP binding; ATPase activity; myosin binding

Biological Process: actin filament-based movement; actin-myosin filament sliding; actomyosin structure organization; apoptosis; cardiac muscle contraction; cardiac muscle morphogensis; cardiac myofibril assembly; heart contraction; muscle filament sliding; muscle thin filament assembly; negative regulation of apoptosis; response to drug; response to ethanol

Disease: Atrial Septal Defect 5; Cardiomyopathy, Dilated, 1r; Cardiomyopathy, Familial Hypertrophic, 11

Research Articles on ACTC1

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Product Notes

The ACTC1 actc1 (Catalog #AAA2090595) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Actin Alpha 1, Cardiac Muscle (ACTC1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Actin Alpha 1 (ACTC1) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/mL Immunocytochemistry in formalin fixed cells: 5-30ug/mL Immunohistochemistry in formalin fixed frozen section: 5-30ug/mL Immunohistochemistry in paraffin section: 5-30ug/mL. Researchers should empirically determine the suitability of the ACTC1 actc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Actin Alpha 1 (ACTC1), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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