Rabbit IP6K2 Polyclonal Antibody | anti-IP6K2 antibody

IP6K2 (L188) Polyclonal Antibody

Cat. Num. AAA3003013

• Gene Names: IP6K2; PIUS; IHPK2; InsP6K2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IP6K2; Polyclonal Antibody; IP6K2 (L188) Polyclonal Antibody; Inositol hexakisphosphate kinase 2; InsP6 kinase 2; P(i)-uptake stimulator; PiUS; IHPK2; TCCCIA00113; anti-IP6K2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IP6K2 (L188) polyclonal antibody detects endogenous levels of IP6K2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 426

• Applicable Applications for anti-IP6K2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 156-204 of Human IP6K2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IP6K2 (L188) pAb at 1:500 dilutionLane1:CT26 whole cell lysate (40ug) Lane2:C6 whole cell lysate (40ug) Lane3:Panc1 whole cell lysate (40ug) Lane4:HCT116 whole cell lysate (40ug) Lane5:A549 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of IP6K2 (L188) pAb in paraffin-embedded human esophageal carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of IP6K2 (L188) pAb in paraffin-embedded human stomach carcinoma tissue at 1:50.showing cytoplasmic and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.)

Related Product Information for anti-IP6K2 antibody

The members of the inositol hexakisphosphate kinase family, IP6K1 and IP6K2, have a high affinity and selectivity for inositol hexakisphosphate (InsP6) as a substrate. IP6K1 and IP6K2 (also designated PiUS) convert InsP6 to PP-InsP5. However, neither kinase demonstrates any catalytic activity with other inositol pyrophosphates. The presence of InsP6, which inhibits serine/threonine protein phosphatases, increases the influx of calcium across the plasma membrane and implies that it may mediate the regulation of insulin exocytosis. IP6K1 was purified as a 54 kDa protein in rat brain extracts. By homology, IP6K1 and IP6K2 were characterized in mouse as a 50 kDa and 49 kDa protein, respectively. IP6K1 displays ATP synthase activity by transferring a phosphate from PP-InsP5 to ADP, which suggests a role for the IP6 kinases as high energy phosphate donors.

NCBI and Uniprot Product Information

• NCBI GI #: 55769520
• NCBI GeneID: 51447
• NCBI Accession #: NP_001005909.1
• NCBI GenBank Nucleotide #: NP_001005909.1
• UniProt Accession #: Q9UHH9
• Molecular Weight: ~ 52kDa
• NCBI Official Full Name: inositol hexakisphosphate kinase 2 isoform a
• NCBI Official Synonym Full Names: inositol hexakisphosphate kinase 2
• NCBI Official Symbol: IP6K2
• NCBI Official Synonym Symbols: PIUS; IHPK2; InsP6K2
• NCBI Protein Information: inositol hexakisphosphate kinase 2
• UniProt Protein Name: Inositol hexakisphosphate kinase 2
• Protein Family: Inositol hexakisphosphate kinase
• UniProt Gene Name: IP6K2
• UniProt Synonym Gene Names: IHPK2; InsP6 kinase 2; PiUS
• UniProt Entry Name: IP6K2_HUMAN

NCBI Description

This gene encodes a protein that belongs to the inositol phosphokinase (IPK) family. This protein is likely responsible for the conversion of inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). It may also convert 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4 and affect the growth suppressive and apoptotic activities of interferon-beta in some ovarian cancers. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

IHPK2: Converts inositol hexakisphosphate (InsP6) to diphosphoinositol pentakisphosphate (InsP7/PP-InsP5). Converts 1,3,4,5,6-pentakisphosphate (InsP5) to PP-InsP4. Belongs to the inositol phosphokinase (IPK) family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 2.7.4.21; Kinase, other

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nucleoplasm; intermediate filament cytoskeleton; nucleus

Molecular Function: inositol hexakisphosphate 5-kinase activity; inositol trisphosphate 3-kinase activity; ATP binding

Biological Process: inositol phosphate metabolic process; phosphate transport; positive regulation of apoptosis; phosphoinositide phosphorylation; cytokine and chemokine mediated signaling pathway; negative regulation of cell growth