Western Blot (WB)
(Western blot analysis of MAK10 in mouse heart tissue lysate with MAK10 antibody at (A) 1 and (B) 2 μg/mL.)
Rabbit MAK10 Polyclonal Antibody | anti-MAK10 antibody
MAK10 Antibody
Gene Names
NAA35; EGAP; MAK10; MAK10P; bA379P1.1
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
MAK10 Antibody is affinity chromatography purified via peptide column.
Synonyms
MAK10; Polyclonal Antibody; MAK10 Antibody; EGAP; MAK10P; bA379P1.1; N-alpha-acetyltransferase 35; NatC auxiliary subunit; Embryonic growth-associated protein homolog; MAK10 homolog; amino-acid N-acetyltransferase subunit (S. cerevisiae); anti-MAK10 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
MAK10 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
725
Applicable Applications for anti-MAK10 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
MAK10 antibody can be used for detection of MAK10 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL.
Conjugate
Unconjugated
Immunogen
MAK10 antibody was raised against a 14 amino acid synthetic peptide near the center of human MAK10.
Buffer
MAK10 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
MAK10 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of MAK10 in mouse heart tissue lysate with MAK10 antibody at (A) 1 and (B) 2 μg/mL.)
Western Blot (WB)
(Western blot analysis of MAK10 in mouse heart tissue lysate with MAK10 antibody at (A) 1 and (B) 2 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of MAK10 in human heart tissue with MAK10 antibody at 5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of MAK10 in human heart tissue with MAK10 antibody at 5 μg/mL.)
Related Product Information for anti-MAK10 antibody
MAK10 Antibody: The MAK10 gene encodes a 733-amino acid protein with several regions of similarity to T cell receptor alpha-subunit V (variable) regions in yeast. The mammalian homologue of yeast MAK10, also known as EGAP, is one subunit of a novel N-terminal acetyltransferase (NAT) that is highly conserved among vertebrate species. It is expressed in a variety of tissues in the developing rat embryo but restricted in expression in the adult, remaining detectable only in tissues undergoing continual cell renewal or in cells responding to pathological injury. The MAK10-NAT complex is an essential regulatory enzyme controlling the function of a subset of proteins required for embryonic growth control and vessel development. This complex functionally co-assembles in mammalian cells to regulate cell proliferation and is essential for embryonic development, at least in part through the regulation of target of rapamycin (TOR) signaling events. At least two isoforms of MAK10 are known to exist.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,165 Da
NCBI Official Full Name
N-alpha-acetyltransferase 35, NatC auxiliary subunit
NCBI Official Synonym Full Names
N(alpha)-acetyltransferase 35, NatC auxiliary subunit
NCBI Official Symbol
NAA35
NCBI Official Synonym Symbols
EGAP; MAK10; MAK10P; bA379P1.1
NCBI Protein Information
N-alpha-acetyltransferase 35, NatC auxiliary subunit; protein MAK10 homolog; corneal wound healing-related protein; embryonic growth-associated protein homolog; MAK10 homolog, amino-acid N-acetyltransferase subunit
UniProt Protein Name
N-alpha-acetyltransferase 35, NatC auxiliary subunit
Protein Family
UniProt Gene Name
NAA35
UniProt Synonym Gene Names
EGAP; MAK10
UniProt Entry Name
NAA35_HUMAN
Uniprot Description
NAA35: Regulates proliferation of smooth muscle cells. Component of the N-terminal acetyltransferase C (NatC) complex which may catalyze acetylation of N-terminal methionine residues. Belongs to the MAK10 family.
Chromosomal Location of Human Ortholog: 9q21.33
Cellular Component: polysome; cytoplasm
Molecular Function: protein binding
Biological Process: smooth muscle cell proliferation; negative regulation of apoptosis
Research Articles on MAK10
Similar Products
Product Notes
The MAK10 naa35 (Catalog #AAA150477) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAK10 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MAK10 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). MAK10 antibody can be used for detection of MAK10 by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. Researchers should empirically determine the suitability of the MAK10 naa35 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAK10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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