Western Blot (WB)
(The anti-MAGEF1 C-term Antibody is used in Western blot to detect MAGEF1 in A375 lysate.)
Rabbit anti-Human MAGEF1 Polyclonal Antibody | anti-MAGEF1 antibody
MAGEF1, CT (Melanoma-associated Antigen F1, MAGE-F1 Antigen) (APC)
Gene Names
MAGEF1; MAGE-F1
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Purified by Protein G Affinity Chromatography.
Synonyms
MAGEF1; Polyclonal Antibody; CT (Melanoma-associated Antigen F1; MAGE-F1 Antigen) (APC); anti-MAGEF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human MAGEF1.
Purity/Purification
Purified by Protein G Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-MAGEF1 antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes
IHC: 1:50-1:100
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human MAGEF1 (KLH).
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(The anti-MAGEF1 C-term Antibody is used in Western blot to detect MAGEF1 in A375 lysate.)
Western Blot (WB)
(The anti-MAGEF1 C-term Antibody is used in Western blot to detect MAGEF1 in A375 lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Product Categories/Family for anti-MAGEF1 antibody
References
1. Stone, B., et al., Gene 267(2):173-182 (2001).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
34kDa
NCBI Official Full Name
melanoma-associated antigen F1
NCBI Official Synonym Full Names
MAGE family member F1
NCBI Official Symbol
MAGEF1
NCBI Official Synonym Symbols
MAGE-F1
NCBI Protein Information
melanoma-associated antigen F1
UniProt Protein Name
Melanoma-associated antigen F1
Protein Family
UniProt Gene Name
MAGEF1
UniProt Entry Name
MAGF1_HUMAN
NCBI Description
This intronless gene encodes a member of the MAGE superfamily. It is ubiquitously expressed in normal tissues and in tumor cells. This gene includes a microsatellite repeat in the coding region. [provided by RefSeq, Jul 2008]
Uniprot Description
MAGE-F1: May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.
Chromosomal Location of Human Ortholog: 3q13
Research Articles on MAGEF1
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Product Notes
The MAGEF1 magef1 (Catalog #AAA6486254) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAGEF1, CT (Melanoma-associated Antigen F1, MAGE-F1 Antigen) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAGEF1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), FLISA, Western Blot (WB). IHC: 1:50-1:100 FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MAGEF1 magef1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGEF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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