Western Blot (WB)
(The anti-MAGEA9 Ctr Antibody is used in Western blot to detect MAGEA9 in Placenta lysate.)
Rabbit anti-Human MAGEA9 Polyclonal Antibody | anti-MAGEA9 antibody
MAGEA9 Antibody (Center)
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
MAGEA9; Polyclonal Antibody; MAGEA9 Antibody (Center); Melanoma-associated antigen 9; Cancer/testis antigen 19; CT19; MAGE-9 antigen; MAGE9; MAGEA9A; anti-MAGEA9 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Clone Number
RB2099
Specificity
This MAGEA9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-198 amino acids from the Central region of human MAGEA9.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Concentration
Vial Concentration: 2 (varies by lot)
Sequence Positions
171-198
Sequence Length
315
Applicable Applications for anti-MAGEA9 antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
IHC-P~~1:50~100
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Function
Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression
Tissue Location
Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta EMBL.
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(The anti-MAGEA9 Ctr Antibody is used in Western blot to detect MAGEA9 in Placenta lysate.)
Western Blot (WB)
(The anti-MAGEA9 Ctr Antibody is used in Western blot to detect MAGEA9 in Placenta lysate.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB)
(Western blot analysis of MAGEA9 (arrow) using rabbit polyclonal MAGEA9 Antibody (C185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAGEA9 gene.)
Western Blot (WB)
(Western blot analysis of MAGEA9 (arrow) using rabbit polyclonal MAGEA9 Antibody (C185). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAGEA9 gene.)
Related Product Information for anti-MAGEA9 antibody
MAGEA9 is a member of the MAGEA gene family. The members of this family have their entire coding sequences located in the last exon, and the encoded proteins show 50 to 80% sequence identity between each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are expressed at a high level in a number of tumors of various histologic types, and are silent in normal tissues with the exception of testis and placenta. The MAGEA genes are clustered on chromosome Xq28. They may be implicated in some hereditary disorders, such as dyskeratosis congenita.
Product Categories/Family for anti-MAGEA9 antibody
References
Rogner, U.C., et al., Genomics 29(3):725-731 (1995).
De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).
De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
melanoma-associated antigen 9
NCBI Official Synonym Full Names
melanoma antigen family A9B
NCBI Official Symbol
MAGEA9B
NCBI Protein Information
melanoma-associated antigen 9
UniProt Protein Name
Melanoma-associated antigen 9
Protein Family
UniProt Gene Name
MAGEA9
UniProt Synonym Gene Names
MAGE9; MAGEA9A; CT1.9
UniProt Entry Name
MAGA9_HUMAN
NCBI Description
MAGEA9B is a duplication of the MAGEA9 gene (MIM 300342) on chromosome Xq28. The 2 copies are separated by about 194 kb (Hartz, 2009).[supplied by OMIM, Mar 2009]
Uniprot Description
MAGE-A9: Not known, though may play a role in embryonal development and tumor transformation or aspects of tumor progression.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: Xq28
Similar Products
Product Notes
The MAGEA9 magea9 (Catalog #AAA9204700) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 171-198. The MAGEA9 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MAGEA9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P). WB~~1:1000 IHC-P~~1:50~100. Researchers should empirically determine the suitability of the MAGEA9 magea9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MAGEA9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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