Mouse anti-Mouse Sox2 Monoclonal Antibody | anti-SOX2 antibody

Sox2 Antibody

Cat. Num. AAA9215547

• Gene Names: SOX2; ANOP3; MCOPS3
• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
• Synonyms: Sox2; Monoclonal Antibody; Sox2 Antibody; Purified Mouse Monoclonal Antibody (Mab); ANOP3; cb236; Delta EF2a; lcc; MCOPS3; MGC148683; MGC2413; RGD1565646; Sex determining region Y box 2; SOX 2; SOX2_HUMAN; SRY (sex determining region Y) box 2; SRY box containing gene 2; SRY related HMG box 2; SRY related HMG box gene 2; SRY-box 2; Transcription factor SOX 2; Transcription factor SOX-2; ysb.; anti-SOX2 antibody

• Host: Mouse
• Reactivity: Mouse
• Clonality: Monoclonal
• Form/Format: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
• Sequence Length: 317

• Applicable Applications for anti-SOX2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: WB ~~ 1:1000; ICC ~~ 1:150; FC ~~ 1:100
• Antigen Source: Mouse
• Antigen Type: Recombinant Protein
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot detection of Sox2 in F9 cell lysates using Sox2 mouse mAb (1:1000 diluted).Predicted band size:35KDa.Observed band size:35KDa.)

Immunocytochemistry (ICC)

(Immunocytochemistry of COS7 cells using anti-Sox2 mouse mAb diluted 1:150.)

Flow Cytometry (FC)

(Flow Cytometry analysis of F9 cells stained with Sox2 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.)

Related Product Information for anti-SOX2 antibody

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).

Product Categories/Family for anti-SOX2 antibody

Developmental Biology; Neuroscience; Stem Cells

References

Stevanovic M.,et al.Mamm. Genome 5:640-642(1994). Sadler L.A.,et al.Submitted (DEC-1992) to the EMBL/GenBank/DDBJ databases. Fantes J.,et al.Nat. Genet. 33:461-463(2003). Takahashi K.,et al.Cell 131:861-872(2007). Rigbolt K.T.,et al.Sci. Signal. 4:RS3-RS3(2011).

NCBI and Uniprot Product Information

• NCBI GI #: 28195386
• NCBI GeneID: 6657
• NCBI Accession #: NP_003097.1
• NCBI GenBank Nucleotide #: NM_003106.3
• UniProt Accession #: P48431; Q14537
• Molecular Weight: 34310
• NCBI Official Full Name: transcription factor SOX-2
• NCBI Official Synonym Full Names: SRY-box 2
• NCBI Official Symbol: SOX2
• NCBI Official Synonym Symbols: ANOP3; MCOPS3
• NCBI Protein Information: transcription factor SOX-2
• UniProt Protein Name: Transcription factor SOX-2
• UniProt Gene Name: SOX2
• UniProt Entry Name: SOX2_HUMAN

NCBI Description

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]

Uniprot Description

SOX2: Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. May function as a switch in neuronal development. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal. Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation. Interacts with ZSCAN10. Interacts with SOX3 and FGFR1.

Protein type: Cell development/differentiation; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 3q26.3-q27

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; miRNA binding; DNA binding; sequence-specific DNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; somatic stem cell maintenance; endodermal cell fate specification; positive regulation of transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; regulation of caspase activity; regulation of transcription, DNA-dependent; positive regulation of MAPKKK cascade; tissue regeneration; forebrain development; response to wounding; positive regulation of cell-cell adhesion; adenohypophysis development; cell cycle arrest; inner ear development; negative regulation of epithelial cell proliferation; osteoblast differentiation; negative regulation of neuron differentiation; eye development; regulation of gene expression; pituitary gland development; glial cell fate commitment; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation

Disease: Microphthalmia, Syndromic 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia