Rabbit anti-Human MAGEA12 Polyclonal Antibody | anti-MAGEA12 antibody

MAGEA12, NT (Melanoma-associated Antigen 12, MAGE-12 Antigen, MAGE-12F Antigen, Cancer/Testis Antigen 1.12, CT1.12, MAGE12) (PE)

Cat. Num. AAA6486307

• Gene Names: MAGEA12; CT1.12; MAGE12
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: MAGEA12; Polyclonal Antibody; NT (Melanoma-associated Antigen 12; MAGE-12 Antigen; MAGE-12F Antigen; Cancer/Testis Antigen 1.12; CT1.12; MAGE12) (PE); anti-MAGEA12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAGEA12.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-MAGEA12 antibody: Immunohistochemistry (IHC), FLISA
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human MAGEA12 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(MAGEA12 Antibody (S96) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the MAGEA12 antibody detected the MAGEA12 protein (arrow).)

Immunohistochemistry (IHC)

(MAGEA12 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAGEA12 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-MAGEA12 antibody

Antibodies; Cancer Markers

References

1. Mallon, A.M., et al., Genome Res. 10(6):758-775 (2000). 2. Rogner, U.C., et al., Genomics 29(3):725-731 (1995). 3. Ding, M., et al., Biochem. Biophys. Res. Commun. 202(1):549-555 (1994). 4. De Smet, C., et al., Immunogenetics 39(2):121-129 (1994). 5. De Plaen, E., et al., Immunogenetics 40(5):360-369 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 58530873
• NCBI GeneID: 4111
• NCBI Accession #: NP_005358
• NCBI GenBank Nucleotide #: NM_005367
• UniProt Accession #: P43365
• Molecular Weight: 35kDa
• NCBI Official Full Name: melanoma-associated antigen 12
• NCBI Official Synonym Full Names: MAGE family member A12
• NCBI Official Symbol: MAGEA12
• NCBI Official Synonym Symbols: CT1.12; MAGE12
• NCBI Protein Information: melanoma-associated antigen 12
• UniProt Protein Name: Melanoma-associated antigen 12
• Protein Family: Melanoma-associated antigen
• UniProt Gene Name: MAGEA12
• UniProt Synonym Gene Names: MAGE12; CT1.12
• UniProt Entry Name: MAGAC_HUMAN

NCBI Description

This gene is closely related to several other genes clustered on chromosome X. These genes may be overexpressed in tumors. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq, Jun 2014]

Uniprot Description

MAGEA12: Not known, though may play a role tumor transformation or progression. In vitro promotes cell viability in melanoma cell lines.

Protein type: Cancer Testis Antigen (CTA)

Chromosomal Location of Human Ortholog: Xq28