Rabbit anti-Human, Mouse GAS7 Polyclonal Antibody | anti-GAS7 antibody

GAS7, CT (Growth Arrest-specific Protein 7, GAS-7, KIAA0394) (HRP)

Cat. Num. AAA6477507

• Gene Names: GAS7; MLL/GAS7
• Reactivity: Human, Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: GAS7; Polyclonal Antibody; CT (Growth Arrest-specific Protein 7; GAS-7; KIAA0394) (HRP); Growth arrest-specific protein 7; KIAA0394; anti-GAS7 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GAS7. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GAS7 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GAS7 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of GAS7 antibody (C-term) in A2058 cell line lysates (35ug/lane). GAS7 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of GAS7 antibody (C-term) in mouse cerebellum tissue lysates (35ug/lane). GAS7 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human brain tissue reacted with GAS7 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(GAS7 Antibody (C-term) flow cytometric analysis of Jurkat cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-GAS7 antibody

Antibodies; Neuroscience

References

1. Chao, C.C., J. Neurosci. Res. 81(2), 153-162 (2005). 2. She, B.R., Exp. Cell Res. 273(1), 34-44 (2002).

NCBI and Uniprot Product Information

• NCBI GI #: 195546919
• NCBI GeneID: 8522
• NCBI Accession #: NP_001124303
• NCBI GenBank Nucleotide #: NM_001130831
• UniProt Accession #: O60861; O43144; Q53Y77; Q7Z571; A8KAC2; B2RCK9
• Molecular Weight: 47,680 Da
• NCBI Official Full Name: growth arrest-specific protein 7 isoform d
• NCBI Official Synonym Full Names: growth arrest-specific 7
• NCBI Official Symbol: GAS7
• NCBI Official Synonym Symbols: MLL/GAS7
• NCBI Protein Information: growth arrest-specific protein 7; MLL/GAS7 fusion protein
• UniProt Protein Name: Growth arrest-specific protein 7
• Protein Family: Growth arrest-specific protein
• UniProt Gene Name: GAS7
• UniProt Synonym Gene Names: KIAA0394; GAS-7
• UniProt Entry Name: GAS7_HUMAN

Uniprot Description

GAS7: May play a role in promoting maturation and morphological differentiation of cerebellar neurons. A chromosomal aberration involving GAS7 is found in acute myeloid leukemia. Translocation t(11;17)(q23;p13) with MLL/HRX. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell development/differentiation; Actin-binding; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 17p13.1

Cellular Component: ruffle; cytoplasm; actin filament

Molecular Function: actin filament binding; transcription factor activity

Biological Process: regulation of cell shape; actin filament bundle formation; regulation of transcription, DNA-dependent; actin filament polymerization; cell cycle arrest; neurite morphogenesis