Goat anti-Human MAD1L1 Polyclonal Antibody | anti-MAD1L1 antibody

MAD1L1 (Mitotic Arrest Deficient 1-Like 1)

Cat. Num. AAA611039

• Gene Names: MAD1L1; MAD1; PIG9; TP53I9; TXBP181
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MAD1L1; Polyclonal Antibody; MAD1L1 (Mitotic Arrest Deficient 1-Like 1); Anti -MAD1L1 (Mitotic Arrest Deficient 1-Like 1); anti-MAD1L1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAD-1L1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile, 0.02% sodium azide or sterile 40-50% glycerol.

• Applicable Applications for anti-MAD1L1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot and ELISA.; Dilution: Western Blot: 1ug/ml
• Immunogen: E. coli-derived recombinant human MAD-1L1 (aa1-350) GenBank Accession #NM_003550
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-MAD1L1 antibody

MAD-1L1 (mitotic arrest deficient 1-like 1) is a member of the spindle-assembly checkpoint (SAC) which prevents anaphase from occurring until all chromosomes are properly aligned at the metaphase plate. Upon checkpoint activation, MAD-1L1 recruits MAD2 to unattached kinetochores, promoting the binding of MAD2 to CDC20, the activator for the anaphase-promoting complex. MAD-1L1 has been implicated to have tumor-suppressing functions, but in small-cell lung cancer cell lines, the MAD-1L1 gene is the most frequent copy number gain, suggesting a more complex role.

Product Categories/Family for anti-MAD1L1 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 62243374
• NCBI GeneID: 8379
• NCBI Accession #: NP_001013859.1
• NCBI GenBank Nucleotide #: NM_001013837.1
• UniProt Accession #: Q9Y6D9; Q13312; Q75MI0; Q86UM4; Q9UNH0
• Molecular Weight: 83,067 Da
• NCBI Official Full Name: mitotic spindle assembly checkpoint protein MAD1
• NCBI Official Synonym Full Names: MAD1 mitotic arrest deficient-like 1 (yeast)
• NCBI Official Symbol: MAD1L1
• NCBI Official Synonym Symbols: MAD1; PIG9; TP53I9; TXBP181
• NCBI Protein Information: mitotic spindle assembly checkpoint protein MAD1; MAD1-like protein 1; tax-binding protein 181; tumor protein p53 inducible protein 9; mitotic checkpoint MAD1 protein homolog; mitotic arrest deficient 1-like protein 1; mitotic-arrest deficient 1, yeast, homolog-like 1
• UniProt Protein Name: Mitotic spindle assembly checkpoint protein MAD1
• Protein Family: Mitotic spindle assembly checkpoint protein
• UniProt Gene Name: MAD1L1
• UniProt Synonym Gene Names: MAD1; TXBP181; MAD1-like protein 1; HsMAD1; hMAD1
• UniProt Entry Name: MD1L1_HUMAN

NCBI Description

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MAD1L1: a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Expressed weakly at G0/G1 and highly at late S and G2/M phase. Induced by p53. Becomes hyperphosphorylated in late S through M phases or after mitotic spindle damage. Two alternatively spliced isoforms have been described. Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.

Protein type: Cell cycle regulation; Cytoskeletal

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: kinetochore; centrosome; cytoplasm; nuclear pore; spindle; nucleus; cytosol; actin cytoskeleton

Molecular Function: protein binding

Biological Process: mitosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle

Disease: Prostate Cancer