Rabbit anti-Human MAD1L1 Polyclonal Antibody | anti-MAD1L1 antibody

MAD1L1, NT (MAD1L1, MAD1, TXBP181, Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, Mitotic checkpoint MAD1 protein homolog, Tax-binding protein 181) (Biotin)

Cat. Num. AAA6317903

• Gene Names: MAD1L1; MAD1; PIG9; TP53I9; TXBP181
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MAD1L1; Polyclonal Antibody; NT (MAD1L1; MAD1; TXBP181; Mitotic spindle assembly checkpoint protein MAD1; Mitotic arrest deficient 1-like protein 1; Mitotic checkpoint MAD1 protein homolog; Tax-binding protein 181) (Biotin); Tax-binding protein 181; anti-MAD1L1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-MAD1L1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: MAD1L1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 131-159 amino acids from the N-terminal region of human MAD1L1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(MAD1L1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the MAD1L1 antibody detected the MAD1L1 protein (arrow).)

Related Product Information for anti-MAD1L1 antibody

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq].

Product Categories/Family for anti-MAD1L1 antibody

Antibodies; Binding Proteins

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Guo, Y., et al. J. Med. Genet. 47(9):616-622(2010). Wang, H.B., et al. J. Gastrointest. Surg. 14(8):1227-1234(2010). Hewitt, L., et al. J. Cell Biol. 190(1):25-34(2010). Ge, Z., et al. FASEB J. 24(2):579-586(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 62243374
• NCBI GeneID: 8379
• NCBI Accession #: NP_001013859.1; NP_001013858.1; NP_003541.2
• NCBI GenBank Nucleotide #: NM_001013837.1
• UniProt Accession #: Q9Y6D9; Q13312; Q75MI0; Q86UM4; Q9UNH0
• Molecular Weight: 83,067 Da
• NCBI Official Full Name: mitotic spindle assembly checkpoint protein MAD1
• NCBI Official Synonym Full Names: MAD1 mitotic arrest deficient-like 1 (yeast)
• NCBI Official Symbol: MAD1L1
• NCBI Official Synonym Symbols: MAD1; PIG9; TP53I9; TXBP181
• NCBI Protein Information: mitotic spindle assembly checkpoint protein MAD1; MAD1-like protein 1; tax-binding protein 181; tumor protein p53 inducible protein 9; mitotic checkpoint MAD1 protein homolog; mitotic arrest deficient 1-like protein 1; mitotic-arrest deficient 1, yeast, h
• UniProt Protein Name: Mitotic spindle assembly checkpoint protein MAD1
• Protein Family: Mitotic spindle assembly checkpoint protein
• UniProt Gene Name: MAD1L1
• UniProt Synonym Gene Names: MAD1; TXBP181; MAD1-like protein 1; HsMAD1; hMAD1
• UniProt Entry Name: MD1L1_HUMAN

NCBI Description

MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

MAD1L1: a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Expressed weakly at G0/G1 and highly at late S and G2/M phase. Induced by p53. Becomes hyperphosphorylated in late S through M phases or after mitotic spindle damage. Two alternatively spliced isoforms have been described. Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.

Protein type: Cytoskeletal; Cell cycle regulation

Chromosomal Location of Human Ortholog: 7p22

Cellular Component: kinetochore; centrosome; cytoplasm; nuclear pore; spindle; nucleus; cytosol; actin cytoskeleton

Molecular Function: protein binding

Biological Process: mitosis; cell division; mitotic cell cycle checkpoint; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle

Disease: Prostate Cancer