Western Blot (WB)
(Western blot analysis of anti-mTLR4 Pab in mouse spleen cell lysate. mTLR4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Mouse M TLR4 Polyclonal Antibody | anti-Tlr4 antibody
M TLR4 Antibody (N-term)
Western Blot (WB)
(Western blot analysis of anti-mTLR4 Pab in mouse spleen cell lysate. mTLR4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-mTLR4 Pab in mouse spleen cell lysate. mTLR4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
This gene belongs to the evolutionarily-conserved Toll-like receptor family, whose members are type-1 transmembrane proteins that are involved in innate immunity. Toll-like receptors are characterized by an extracellular leucine-rich repeat domain that functions in ligand recognition and an intracellular toll/interleukin-1 receptor-like domain that is crucial for signal transduction. The receptor encoded by this gene mediates the innate immune response to bacterial lipopolysaccharide, a major component of the outer membrane of Gram-negative bacteria, through synthesis of pro-inflammatory cytokines and chemokines. In addition, this protein can recognize other pathogens from Gram-negative and Gram-positive bacteria as well as viral components. Mice deficient in this gene display a number of immune response-related phenotypes including hyporesponsiveness to bacterial lipopolysaccharide and increased levels of respiratory syncytial virus compared to controls. [provided by RefSeq, Sep 2015]
Uniprot Description
TLR4: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Also involved in LPS- independent inflammatory responses triggered by Ni(2+). These responses require non-conserved histidines and are, therefore, species-specific. Belongs to the lipopolysaccharide (LPS) receptor, a multi-protein complex containing at least CD14, LY96 and TLR4. Binding to bacterial LPS leads to homodimerization. Interacts with LY96 via the extracellular domain. Interacts with MYD88 and TIRAP via their respective TIR domains. Interacts with NOX4. Interacts with CNPY3. Interacts with HSP90B1. The interaction with both CNPY3 and HSP90B1 is required for proper folding in the endoplasmic reticulum. Highly expressed in placenta, spleen and peripheral blood leukocytes. Detected in monocytes, macrophages, dendritic cells and several types of T-cells. Belongs to the Toll-like receptor family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Cellular Component: cell surface; membrane; perinuclear region of cytoplasm; integral to plasma membrane; cytoplasm; integral to membrane; plasma membrane; lipopolysaccharide receptor complex; lipid raft; external side of plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; lipopolysaccharide binding; lipopolysaccharide receptor activity; phosphoinositide 3-kinase binding; receptor activity
Biological Process: positive regulation of nitric oxide biosynthetic process; activation of MAPK activity; positive regulation of apoptosis; response to lipopolysaccharide; leukotriene metabolic process; positive regulation of NF-kappaB import into nucleus; positive regulation of interleukin-10 production; activation of NF-kappaB transcription factor; negative regulation of interleukin-6 production; positive regulation of B cell proliferation; positive regulation of interferon-beta production; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; positive regulation of DNA binding; positive regulation of I-kappaB kinase/NF-kappaB cascade; production of nitric oxide during acute inflammatory response; positive regulation of interleukin-6 production; positive regulation of interleukin-8 biosynthetic process; positive regulation of tumor necrosis factor production; positive regulation of chemokine production; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of interleukin-23 production; macrophage activation; response to ethanol; response to bacterium; detection of lipopolysaccharide; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; response to oxidative stress; I-kappaB phosphorylation; activation of innate immune response; positive regulation of nitric-oxide synthase biosynthetic process; regulation of cytokine secretion; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; microglial cell activation; signal transduction; defense response to Gram-negative bacterium; mast cell activation; positive regulation of stress-activated MAPK cascade; positive regulation of interleukin-1 biosynthetic process; positive regulation of interleukin-8 production; positive regulation of lymphocyte proliferation; negative regulation of interferon-gamma production; positive regulation of interleukin-13 biosynthetic process; positive regulation of MHC class II biosynthetic process; interferon-gamma production; lipopolysaccharide-mediated signaling pathway; inflammatory response; innate immune response-activating signal transduction; B cell proliferation during immune response; positive regulation of tumor necrosis factor biosynthetic process; immune system process; activation of NF-kappaB-inducing kinase; negative regulation of tumor necrosis factor production; MyD88-dependent toll-like receptor signaling pathway; positive regulation of interferon-gamma production; positive regulation of interleukin-12 biosynthetic process; negative regulation of interleukin-17 production; positive regulation of interferon-beta biosynthetic process; regulation of inflammatory response; toll-like receptor signaling pathway; innate immune response; immune response; regulation of sensory perception of pain; positive regulation of interleukin-1 production; positive regulation of inflammatory response
Research Articles on Tlr4
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Product Notes
The Tlr4 tlr4 (Catalog #AAA9203360) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 25-55. The M TLR4 Antibody (N-term) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's M TLR4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Tlr4 tlr4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "M TLR4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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