Testing Data
Rabbit anti-Rat LP-82 Polyclonal Antibody | anti-LP-82 antibody
Anti-LP-82 (Lens-specific Calpain) and LP-85, Domain-III, Large Subunit Antibody
Reactivity
Rat
Applications
Western Blot
Purity
Affinity Purified
Synonyms
LP-82; Polyclonal Antibody; Anti-LP-82 (Lens-specific Calpain) and LP-85; Domain-III; Large Subunit Antibody; Calpain; anti-LP-82 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
The antibody is based on the rat sequence. The antibody binds to LP82-calpain and LP-85 calpain, but does not cross react with the other calpain family members (m-calpain, M-calpain, calpain-94, ncl-2, ncl-3, etc.).
Purity/Purification
Affinity Purified
Form/Format
Liquid; PBS, pH 7.4 with 0.05% sodium azide.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-LP-82 antibody
Western Blot (WB)
Application Notes
LP82-Calpain, also known as lens-specific calpain (LP=lens protein), is an intracellular, calcium-dependent cysteine protease. LP-85 is identical to LP-82 calpain, except for the insert that distinguishes LP-85. The calpains have papain-like activity, thus the-pain nomenclature. Unlike M-calpain and m-calpain, which are ubiquitously expressed, LP-82 calpain was originally identified in rat lens. The human sequence for LP-82 has a mutation producing a stop codon, and is not thought to be expressed. Like the other calpains, the endogenous calpain inhibitor, calpastatin, counters LP82. Because of the limited distribution and sequence differences, LP-82 calpain is thought to have different roles than the other calpain family members. The calpain family members consist of a common small subunit, and a large variable subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV. RP1-LP82/85-Calpain recognizes latent and active LP82-calpain and LP-85 calpain. The latent large subunits are 85 Kd and 82 Kd, and the amino terminal truncation at activation yields an approximately 60-62 Kd form. A cascade of smaller forms can be seen with further activation. RP1-LP82/85-Calpain works well for Western Blotting applications, and when used against the reduced protein identifies bands at 85 Kd, 82 Kd, 62 Kd, 60 Kd, and a series of further cleaved active forms. RP1-LP85-Calpain will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. LP 82 and LP85 calpain are cellular proteins, thus cell lysates work well for Western blots. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species.
Immunogen
Synthetic peptide based on the carboxy-terminal end of the domain-III from LP-82-calpain large subunit).
Preparation and Storage
This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
For extended storage, aliquot contents and freeze at-20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.
Testing Data
Testing Data
Related Product Information for anti-LP-82 antibody
LP82/85-calpain is a Rabbit antibody to the cysteine protease, LP82-calpain, made with a synthetic peptide based on the carboxy-terminal end of the domain-III from LP-82-calpain large subunit). The antibody is based on the rat sequence. The antibody binds to LP82-calpain and LP-85 calpain, but does not cross react with the other calpain family members (m-calpain, M-calpain, calpain-94, ncl-2, ncl-3, etc.). The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of Sodium Azide as preservative, and 50% glycerol as cryoprotectant
Similar Products
Product Notes
The LP-82 (Catalog #AAA4157399) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LP-82 (Lens-specific Calpain) and LP-85, Domain-III, Large Subunit Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LP-82 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). LP82-Calpain, also known as lens-specific calpain (LP=lens protein), is an intracellular, calcium-dependent cysteine protease. LP-85 is identical to LP-82 calpain, except for the insert that distinguishes LP-85. The calpains have papain-like activity, thus the-pain nomenclature. Unlike M-calpain and m-calpain, which are ubiquitously expressed, LP-82 calpain was originally identified in rat lens. The human sequence for LP-82 has a mutation producing a stop codon, and is not thought to be expressed. Like the other calpains, the endogenous calpain inhibitor, calpastatin, counters LP82. Because of the limited distribution and sequence differences, LP-82 calpain is thought to have different roles than the other calpain family members. The calpain family members consist of a common small subunit, and a large variable subunit. Domains in the large subunit include the amino terminal domain-I, the proteinase domain-II, domain-III, and the EF-hand domain-IV. RP1-LP82/85-Calpain recognizes latent and active LP82-calpain and LP-85 calpain. The latent large subunits are 85 Kd and 82 Kd, and the amino terminal truncation at activation yields an approximately 60-62 Kd form. A cascade of smaller forms can be seen with further activation. RP1-LP82/85-Calpain works well for Western Blotting applications, and when used against the reduced protein identifies bands at 85 Kd, 82 Kd, 62 Kd, 60 Kd, and a series of further cleaved active forms. RP1-LP85-Calpain will also bind to the non-reduced protein, and thus has application for immunoprecipitation, immunohistochemistry, and ELISA. LP 82 and LP85 calpain are cellular proteins, thus cell lysates work well for Western blots. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. Researchers should empirically determine the suitability of the LP-82 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LP-82, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
