Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human Raji Cells.)
Rabbit anti-Human Lymphocyte Function Associated Antigen 1 Alpha Polyclonal Antibody | anti-LFA1a antibody
Polyclonal Antibody to Lymphocyte Function Associated Antigen 1 Alpha (LFA1a)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NL EEDAYWVQLD LHFPPGLSFR KVEMLKPHSQ IPVSCEELPE ESRLLSRALS CNVSSPIFKA GHSVALQMMF NTLVNSSWGD SVELHANVTC NNEDSDLLED NSATTIIPIL YPINILIQDQ EDSTLYVSFT PKGPKIHQVK HMYQVRIQPS IHDHNIPTLE AVVGVPQPPS EGPITHQWSV QMEPPVPCHY EDLERLPDAA EPCLPGALFR CPVVFRQEIL VQVIGTLELV GEIEASSMFS LCSSLSISFN
Immunocytochemistry in formalin fixed cells: 5-20ug/mL
Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL
Immunohistochemistry in paraffin section: 5-20ug/mL
Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human Raji Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human Jurkat Cells; Lane2: Human Raji Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant LFA1a, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant LFA1a, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
NCBI Description
ITGAL encodes the integrin alpha L chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form the integrin lymphocyte function-associated antigen-1 (LFA-1), which is expressed on all leukocytes. LFA-1 plays a central role in leukocyte intercellular adhesion through interactions with its ligands, ICAMs 1-3 (intercellular adhesion molecules 1 through 3), and also functions in lymphocyte costimulatory signaling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
ITGAL: Integrin alpha-L/beta-2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4. It is involved in a variety of immune phenomena including leukocyte-endothelial cell interaction, cytotoxic T-cell mediated killing, and antibody dependent killing by granulocytes and monocytes. Belongs to the integrin alpha chain family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Cell surface; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: cell surface; integrin complex; membrane; plasma membrane
Molecular Function: cell adhesion molecule binding; ICAM-3 receptor activity; metal ion binding; protein binding; protein complex binding; protein heterodimerization activity
Biological Process: cell adhesion; cell motility; cell-matrix adhesion; extracellular matrix organization; heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; inflammatory response; integrin-mediated signaling pathway; leukocyte adhesion; leukocyte migration; neutrophil degranulation; phagocytosis; receptor clustering; regulation of immune response; signal transduction; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
Research Articles on LFA1a
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Product Notes
The LFA1a itgal (Catalog #AAA2026363) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Lymphocyte Function Associated Antigen 1 Alpha (LFA1a) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Lymphocyte Function Associated Antigen 1 Alpha can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 1-5ug/mL Immunocytochemistry in formalin fixed cells: 5-20ug/mL Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL Immunohistochemistry in paraffin section: 5-20ug/mL Enzyme-linked Immunosorbent Assay: 0.05-2ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the LFA1a itgal for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NL EEDAYWVQLD LHFPPGLSFR KVEMLKPHSQ IPVSCEELPE ESRLLSRALS CNVSSPIFKA GHSVALQMMF NTLVNSSWGD SVELHANVTC NNEDSDLLED NSATTIIPIL YPINILIQDQ EDSTLYVSFT PKGPKIHQVK HMYQVRIQPS IHDHNIPTLE AVVGVPQPPS EGPITHQWSV QMEPPVPCHY EDLERLPDAA EPCLPGALFR CPVVFRQEIL VQVIGTLELV GEIEASSMFS LCSSLSISFN. It is sometimes possible for the material contained within the vial of "Lymphocyte Function Associated Antigen 1 Alpha, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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