Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Lymphocyte Antigen 6 Complex Antibody. The lane on the left was treated with blocking peptide.)
Rabbit anti-Human, Mouse Lymphocyte Antigen 6 Complex Polyclonal Antibody | anti-LY6K antibody
Lymphocyte Antigen 6 Complex Antibody
Gene Names
LY6K; CT97; ly-6K; URLC10; HSJ001348
Reactivity
Human, Mouse
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
Lymphocyte Antigen 6 Complex; Polyclonal Antibody; Lymphocyte Antigen 6 Complex Antibody; Lymphocyte antigen Ly-6K; 2410015A16Rik; 3110035B01Rik; CT97; FLJ35226; HSJ001348; ly 6K; Lymphocyte antigen 6 complex; locus K; mLy 6K; ThB; Thymocyte B cell antigen; anti-LY6K antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Lymphocyte Antigen 6 Complex Antibody detects endogenous levels of total Lymphocyte Antigen 6 Complex.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
223
Applicable Applications for anti-LY6K antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human Lymphocyte Antigen 6 Complex, corresponding to a region within N-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Lymphocyte Antigen 6 Complex Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Lymphocyte Antigen 6 Complex Antibody. The lane on the left was treated with blocking peptide.)
Immunohistochemistry (IHC)-Paraffin
(MBS9611975 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9611975 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Related Product Information for anti-LY6K antibody
Function: Required for sperm migration into the oviduct and male fertility by controlling binding of sperm to zona pellucida (By similarity). May play a role in cell growth (PubMed:18089789).
Subcellular Location: Secreted. Cytoplasm. Cell membrane>Lipid-anchor. Cytoplasmic vesicle>Secretory vesicle>Acrosome. Membrane raft.
Tissue Specificity: Specifically expressed in testis (at protein level).
Subunit Structure: Interacts with TEX101.
Subcellular Location: Secreted. Cytoplasm. Cell membrane>Lipid-anchor. Cytoplasmic vesicle>Secretory vesicle>Acrosome. Membrane raft.
Tissue Specificity: Specifically expressed in testis (at protein level).
Subunit Structure: Interacts with TEX101.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)18kD.
Predicted Molecular Weight: (Calculated)19kDa.
Predicted Molecular Weight: (Calculated)19kDa.
NCBI Official Full Name
lymphocyte antigen 6 complex, locus K, isoform CRA_b
NCBI Official Synonym Full Names
lymphocyte antigen 6 family member K
NCBI Official Symbol
LY6K
NCBI Official Synonym Symbols
CT97; ly-6K; URLC10; HSJ001348
NCBI Protein Information
lymphocyte antigen 6K
UniProt Protein Name
Lymphocyte antigen 6K
Protein Family
UniProt Gene Name
LY6K
UniProt Synonym Gene Names
CO16; Ly-6K
UniProt Entry Name
LY6K_HUMAN
Uniprot Description
LY6K: May play a role in cell growth.
Protein type: Cancer Testis Antigen (CTA); Membrane protein, GPI anchor
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: cytoplasm; plasma membrane; extracellular region; nucleolus; nucleus
Research Articles on LY6K
Similar Products
Product Notes
The LY6K ly6k (Catalog #AAA9611975) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Lymphocyte Antigen 6 Complex Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Lymphocyte Antigen 6 Complex can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the LY6K ly6k for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lymphocyte Antigen 6 Complex, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
