Monkey Ribosomal Protein L10 ELISA Kit | RPL10 elisa kit

Monkey Ribosomal Protein L10 ELISA Kit

Cat. Num. AAA735255

• Gene Names: RPL10; QM; L10; NOV; AUTSX5; DXS648; DXS648E
• Reactivity: Monkey
• Synonyms: Ribosomal Protein L10; Monkey Ribosomal Protein L10 ELISA Kit; RPL10 elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This RPL10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey RPL10. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for RPL10 elisa kit

Principle of the assay: RPL10 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for RPL10. Standards or samples are then added to the microtiter plate wells and RPL10 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of RPL10 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for RPL10 are added to each well to “sandwich” the RPL10 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain RPL10 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPL10 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for RPL10 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 49457091
• NCBI GeneID: 6134
• UniProt Accession #: P27635; Q16470; Q2HXT7; Q53FH7; Q6FGN8; Q8TDA5; A3KQT0; D3DWW6; Q5HY50; Q9GZW2
• Molecular Weight: 24,604 Da
• NCBI Official Full Name: RPL10
• NCBI Official Synonym Full Names: ribosomal protein L10
• NCBI Official Symbol: RPL10
• NCBI Official Synonym Symbols: QM; L10; NOV; AUTSX5; DXS648; DXS648E
• NCBI Protein Information: 60S ribosomal protein L10; tumor suppressor QM; laminin receptor homolog; Wilms tumor-related protein
• UniProt Protein Name: 60S ribosomal protein L10
• Protein Family: 60S ribosomal protein
• UniProt Gene Name: RPL10
• UniProt Synonym Gene Names: DXS648E; QM
• UniProt Entry Name: RL10_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L10E family of ribosomal proteins. It is located in the cytoplasm. In vitro studies have shown that the chicken protein can bind to c-Jun and can repress c-Jun-mediated transcriptional activation, but these activities have not been demonstrated in vivo. This gene was initially identified as a candidate for a Wilms tumor suppressor gene, but later studies determined that this gene is not involved in the suppression of Wilms tumor. This gene has been referred to as 'laminin receptor homolog' because a chimeric transcript consisting of sequence from this gene and sequence from the laminin receptor gene was isolated; however, it is not believed that this gene encodes a laminin receptor. Alternative splicing results in multiple transcript variants. This gene also uses multiple polyA signals, with the 3'-most polyA signal overlapping the deoxyribonuclease I-like 1 gene on the opposite strand. This gene is co-transcribed with the small nucleolar RNA gene U70, which is located in its fifth intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Feb 2012]

Uniprot Description

RPL10: Defects in RPL10 are a cause of susceptibility to autism X-linked type 5 (AUTSX5). A complex multifactorial, pervasive developmental disorder characterized by impairments in reciprocal social interaction and communication, restricted and stereotyped patterns of interests and activities, and the presence of developmental abnormalities by 3 years of age. Most individuals with autism also manifest moderate mental retardation. RPL10 is involved in autism only in rare cases. Two hypomorphic variants affecting the translation process have been found in families with autism spectrum disorders, suggesting that aberrant translation may play a role in disease mechanisms. Belongs to the ribosomal protein L10e family.

Protein type: Ribosomal; Translation

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: membrane; endoplasmic reticulum; cytosol

Molecular Function: protein binding; structural constituent of ribosome

Biological Process: SRP-dependent cotranslational protein targeting to membrane; cellular protein metabolic process; translational elongation; viral reproduction; translation; translational initiation; mRNA catabolic process, nonsense-mediated decay; gene expression; viral transcription; viral infectious cycle; translational termination

Disease: Autism, Susceptibility To, X-linked 5