Immunohistochemistry (IHC)
(MBS9603081 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit LT4R1 Polyclonal Antibody | anti-LT4R1 antibody
LT4R1 Antibody
Gene Names
LTB4R; BLT1; BLTR; P2Y7; GPR16; LTBR1; P2RY7; CMKRL1; LTB4R1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Peptide affinity purification
Synonyms
LT4R1; Polyclonal Antibody; LT4R1 Antibody; BLT; BLT1; BLTR; Chemoattractant receptor-like 1; Chemokine receptor like 1; CMKRL1; G protein coupled receptor 16; G-protein coupled receptor 16; GPR16; Leukotriene B4 G Protein coupled receptor; Leukotriene B4 receptor 1; Leukotriene B4 receptor; LT4R1_HUMAN; LTB4 R 1; LTB4-R 1; LTB4-R1; LTB4R 1; Ltb4r; LTB4R1; LTBR1; P2RY7; P2Y purinoceptor 7; P2Y7; Purinergic receptor P2Y G protein coupled 7; anti-LT4R1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Monkey
Clonality
Polyclonal
Specificity
LT4R1 Antibody detects endogenous levels of total LT4R1.
Purity/Purification
Peptide affinity purification
Form/Format
IgG in phosphate buffered saline , pH 7.4, 150mMNaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-LT4R1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (peptide)
Application Notes
WB 1:1000-3000, IHC 1:200, ELISA(peptide)1:20000-1:40000
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
*The optimal dilutions should be determined by the end user.
IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
Immunogen
A synthesized peptide derived from human LT4R1,corresponding to a region within the internal amino acids.
Preparation and Storage
Store at -20°C.Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9603081 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9603081 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of extracts from VERO, using LT4R1 Antibody. The lane on the left was treated with blocking peptide)
Western Blot (WB)
(Western blot analysis of extracts from VERO, using LT4R1 Antibody. The lane on the left was treated with blocking peptide)
Related Product Information for anti-LT4R1 antibody
Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38 kDa
NCBI Official Full Name
leukotriene B4 receptor 1
NCBI Official Synonym Full Names
leukotriene B4 receptor
NCBI Official Symbol
LTB4R
NCBI Official Synonym Symbols
BLT1; BLTR; P2Y7; GPR16; LTBR1; P2RY7; CMKRL1; LTB4R1
NCBI Protein Information
leukotriene B4 receptor 1
UniProt Protein Name
Leukotriene B4 receptor 1
UniProt Gene Name
LTB4R
UniProt Synonym Gene Names
BLT; BLT1; BLTR; CMKRL1; GPR16; P2RY7; LTB4-R 1; LTB4-R1; P2Y7
Uniprot Description
Receptor for extracellular ATP > UTP and ADP. The activity of this receptor is mediated by G proteins which activate a phosphatidylinositol-calcium second messenger system. May be the cardiac P2Y receptor involved in the regulation of cardiac muscle contraction through modulation of L-type calcium currents. Is a receptor for leukotriene B4, a potent chemoattractant involved in inflammation and immune response.
Research Articles on LT4R1
Similar Products
Product Notes
The LT4R1 ltb4r (Catalog #AAA9603081) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LT4R1 Antibody reacts with Human, Mouse, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's LT4R1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (peptide). WB 1:1000-3000, IHC 1:200, ELISA(peptide)1:20000-1:40000 *The optimal dilutions should be determined by the end user. IMPORTANT: For western blot, incubate membrane with diluted primary Abin 5% w/v milk, 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight. Researchers should empirically determine the suitability of the LT4R1 ltb4r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LT4R1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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