Rabbit anti-Human SGPP1 Polyclonal Antibody | anti-SGPP1 antibody

SGPP1, NT (SGPP1, Sphingosine-1-phosphate phosphatase 1, Sphingosine-1-phosphatase 1) (PE)

Cat. Num. AAA6347075

• Gene Names: SGPP1; SPPase1
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SGPP1; Polyclonal Antibody; NT (SGPP1; Sphingosine-1-phosphate phosphatase 1; Sphingosine-1-phosphatase 1) (PE); Sphingosine-1-phosphatase 1; anti-SGPP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-SGPP1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SGPP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human SGPP1.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SGPP1 Antibody (N-term) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the SGPP1 antibody detected the SGPP1 protein (arrow).)

Immunohistochemistry (IHC)

(SGPP1 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SGPP1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SGPP1 antibody

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM].

Product Categories/Family for anti-SGPP1 antibody

Antibodies; Enzymes; Phosphatase

References

Hicks, A.A., et al. PLoS Genet. 5 (10), E1000672 (2009): Le Stunff, H., et al. J. Biol. Chem. 282(47):34372-34380(2007). Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007). Olsen, J.V., et al. Cell 127(3):635-648(2006). Giussani, P., et al. Mol. Cell. Biol. 26(13):5055-5069(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 13540569
• NCBI GeneID: 81537
• NCBI Accession #: NP_110418.1
• NCBI GenBank Nucleotide #: NM_030791.2
• UniProt Accession #: Q9BX95; Q9H189; B2RAH0
• Molecular Weight: 49,108 Da
• NCBI Official Full Name: sphingosine-1-phosphate phosphatase 1
• NCBI Official Synonym Full Names: sphingosine-1-phosphate phosphatase 1
• NCBI Official Symbol: SGPP1
• NCBI Official Synonym Symbols: SPPase1
• NCBI Protein Information: sphingosine-1-phosphate phosphatase 1; spp1; hSPP1; hSPPase1; sphingosine-1-phosphatase 1
• UniProt Protein Name: Sphingosine-1-phosphate phosphatase 1
• Protein Family: Sphingosine-1-phosphate phosphatase
• UniProt Gene Name: SGPP1
• UniProt Synonym Gene Names: SPPase1; Spp1; hSPP1; hSPPase1
• UniProt Entry Name: SGPP1_HUMAN

NCBI Description

Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid metabolite that regulates diverse biologic processes. SGPP1 catalyzes the degradation of S1P via salvage and recycling of sphingosine into long-chain ceramides (Mandala et al., 2000 [PubMed 10859351]; Le Stunff et al., 2007 [PubMed 17895250]).[supplied by OMIM, Jun 2009]

Uniprot Description

SGPP1: Has enzymatic activity against both sphingosine 1- phosphate (S1P) and dihydro-S1P. Regulates intracellular and extracellular S1P levels. Belongs to the type 2 lipid phosphate phosphatase family.

Protein type: Mitochondrial; Motility/polarity/chemotaxis; Membrane protein, multi-pass; Endoplasmic reticulum; Apoptosis; Phosphatase (non-protein); Cytoskeletal; Lipid Metabolism - sphingolipid; Membrane protein, integral; EC 3.1.3.-

Chromosomal Location of Human Ortholog: 14q23.2

Cellular Component: endoplasmic reticulum membrane; integral to membrane

Molecular Function: sphingosine-1-phosphate phosphatase activity

Biological Process: sphinganine-1-phosphate metabolic process; sphingolipid metabolic process; sphingolipid biosynthetic process; dephosphorylation; sphingosine metabolic process