Goat LSP1 Polyclonal Antibody | anti-LSP1 antibody

Goat Polyclonal to Human LSP1

Cat. Num. AAA247846

• Gene Names: LSP1; WP34; pp52
• Reactivity: Dog, Gorilla, Hamster, Human, Monkey, Mouse, Rat; Predicted Reactivity: Bovine (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: LSP1; Polyclonal Antibody; Goat Polyclonal to Human LSP1; Anti-LSP1 Antibody (C-Terminus) IHC-plus; 47 kDa actin-binding protein; 47 kDa actin binding protein; 52 kDa phosphoprotein; Leukocyte-specific protein 1; Lymphocyte-specific protein 1; Pp52; WP34; Human LSP1; anti-LSP1 antibody

• Host: Goat
• Reactivity: Dog, Gorilla, Hamster, Human, Monkey, Mouse, Rat; Predicted Reactivity: Bovine (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human LSP1. This antibody is expected to recognize both reported isoforms (NP_002330.1; NP_001013271.1). Reported variants represent identical protein: NP_001013273.1, NP_001013271.1, NP_001013272.1
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 339

• Applicable Applications for anti-LSP1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:16000), IHC-P (3.75 ug/ml), WB (0.05 - 0.2 ug/ml); Usage: Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: Approx 50kDa band observed in Human Lymph Node, Tonsil and Peripheral Blood Mononucleocytes lysates (calculated MW of 37.2kDa according to NP_002330.1). The observed molecular we...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-RYKFVATGHGKYEKV from the C-terminus of human LSP1 (NP_002330.1; NP_001013271.1). Percent identity by BLAST analysis: Human, Gorilla, Baboon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog (100%); Bovine (93%).
• Immunogen Type: Synthetic peptide
• Immunogen: LSP1 antibody was raised against synthetic peptide C-RYKFVATGHGKYEKV from the C-terminus of human LSP1 (NP_002330.1; NP_001013271.1). Percent identity by BLAST analysis: Human, Gorilla, Baboon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog (100%); Bovine (93%); Xenopus, Pufferfish, Zebrafish (87%); Stickleback (80%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-LSP1 antibody IHC of human colon, MALT. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3.75 ug/ml.)

Western Blot (WB)

(LSP1 antibody (0.05 ug/ml) staining of Human Peripheral Lymphocytes lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

NCBI and Uniprot Product Information

• NCBI GI #: 10880979
• NCBI GeneID: 4046
• NCBI Accession #: NP_002330.1
• NCBI GenBank Nucleotide #: NM_002339.2
• UniProt Accession #: P33241; Q16096; Q53H48; Q6FHM3; Q9BUY8; B3KPP1; B3KRR6; E9PBV6; E9PFP3
• Molecular Weight: 50,389 Da
• NCBI Official Full Name: lymphocyte-specific protein 1 isoform 1
• NCBI Official Synonym Full Names: lymphocyte-specific protein 1
• NCBI Official Symbol: LSP1
• NCBI Official Synonym Symbols: WP34; pp52
• NCBI Protein Information: lymphocyte-specific protein 1; 52 kDa phosphoprotein; 47 kDa actin binding protein; 47 kDa actin-binding protein; leukocyte-specific protein 1; lymphocyte-specific antigen WP34; leufactin (leukocyte F-actin binding protein); F-actin binding and cytoskeleton associated protein
• UniProt Protein Name: Lymphocyte-specific protein 1
• Protein Family: Lymphocyte-specific protein
• UniProt Gene Name: LSP1
• UniProt Synonym Gene Names: WP34; pp52
• UniProt Entry Name: LSP1_HUMAN

NCBI Description

This gene encodes an intracellular F-actin binding protein. The protein is expressed in lymphocytes, neutrophils, macrophages, and endothelium and may regulate neutrophil motility, adhesion to fibrinogen matrix proteins, and transendothelial migration. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

LSP1: May play a role in mediating neutrophil activation and chemotaxis. Binds actin. Activated T-lymphocytes.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: membrane; plasma membrane; actin cytoskeleton

Molecular Function: signal transducer activity; actin binding

Biological Process: cellular defense response; chemotaxis; cell motility; signal transduction