Western Blot (WB)
(Western blot analysis of extract from control 293 cells (Lane 1) or transfected with His-tagged S6 (Lane 2) and transfected with His-tagged CREB (Lane 3) using His-Tag polyclonal antibody.)
anti-All His-Tag Polyclonal Antibody
His-Tag Polyclonal Antibody
Reactivity
All
Applications
Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Synonyms
His-Tag; Polyclonal Antibody; His-Tag Polyclonal Antibody; anti-His-Tag antibody
Reactivity
All
Clonality
Polyclonal
Specificity
His-Tag polyclonal antibody detects His-tagged proteins overexpressed in cells. The antibody recognizes the His-tag fused to either the amino-or carboxy-terminus of targeted proteins in transfected mammalian cells.
Purity/Purification
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific peptide.
Form/Format
In phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Applicable Applications for anti-His-Tag antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
The antiserum was produced by immunizing rabbit with synthesized peptide containing the His epitope (H-H-H-H-H-H).
Preparation and Storage
Store at-20 degree C for 1 year
Western Blot (WB)
(Western blot analysis of extract from control 293 cells (Lane 1) or transfected with His-tagged S6 (Lane 2) and transfected with His-tagged CREB (Lane 3) using His-Tag polyclonal antibody.)
Western Blot (WB)
(Western blot analysis of extract from control 293 cells (Lane 1) or transfected with His-tagged S6 (Lane 2) and transfected with His-tagged CREB (Lane 3) using His-Tag polyclonal antibody.)
References
Terpe K. et al. (2003) Appl Microbiol Biotechnol; 60(5):523-533. Xu L. et al. (2006) Cell Mol Immunol; 3(2):139-143. Els Conrath, K., et al. (2001) J. Biol. Chem. 276: 7346-7350.
Similar Products
Product Notes
The His-Tag (Catalog #AAA8509302) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The His-Tag Polyclonal Antibody reacts with All and may cross-react with other species as described in the data sheet. AAA Biotech's His-Tag can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the His-Tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His-Tag, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
