Mouse CDw78 Monoclonal Antibody | anti-CDw78 antibody

CDw78

Cat. Num. AAA4380395

• Reactivity: Human. Others not tested.
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Synonyms: CDw78; Monoclonal Antibody; Leu21; Ba antigen; LO panBa; anti-CDw78 antibody

• Host: Mouse
• Reactivity: Human. Others not tested.
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: DF1588
• Specificity: Reacts with a carbohydrate determinant, identified as CDw78 (Workshop V; Code CDw78.2). Epitope recognized by this MAb and Leu21 is the same. It is present on some immature and some peripheral blood B cells, and some B cell lines. Its expression is increased after B-cell activation in vitro. CDw78 is also found on tissue macrophages and on epithelial cells, but not on T cells, NK cells, monocytes, granulocytes and myeloid tissues. It appears that CDw78 is a determinant on a major histocompatibility complex (MHC) class II subpopulation.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

• Applicable Applications for anti-CDw78 antibody: Functional Studies, Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Functional Studies: Order Ab without Azide.; FC/FACS: 0.5-1ug/million cells; IF: 0.5-1ug/ml
• Immunogen: Human leucocytes
• Cellular Localization: Cell Surface
• Positive Control: Raji and human peripheral blood lymphocytes. Human tonsil and lymph node.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C.; Antibody without azide - store at -20 to -80 degree C.; Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Double Stranded DNA Monoclonal Antibody (AE-2))

References

Rasmussen AM et al. CDw78--a determinant on a major histocompatibility complex class II subpopulation that can be induced to associate with the cytoskeleton. Eur J Immunol 1997, 27(12):3206-3213. Slack JL et al. Molecular characterization of the pan-B cell antigen CDw78 as a MHC class II molecule by direct expression cloning of the transcription factor CIITA. Int Immunol 1995, 7(7):1087-1092. Kikutani H et al. Expression and function of an early activation marker restricted to human B cells. J Immunol 1986, 136(11):4019-4026. Schlossman SF et al. Leukocyte Typing V, p604-606, Oxford University Press, Oxford, 1995. Knapp W et al. Leukocyte Typing IV, p122, p155-162 and p165-174, Oxford University Press, Oxford, 1989.