Rabbit LSD1 Polyclonal Antibody | anti-KDM1 antibody

LSD1 Antibody

Cat. Num. AAA150364

• Gene Names: KDM1A; AOF2; KDM1; LSD1; BHC110
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: LSD1 Antibody is affinity chromatography purified via peptide column.
• Synonyms: LSD1; Polyclonal Antibody; LSD1 Antibody; AOF2; KDM1; BHC110; KIAA0601; Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; lysine (K)-specific demethylase 1; anti-KDM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: LSD1 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 876

• Applicable Applications for anti-KDM1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: LSD1 antibody can be used for the detection of LSD1 by Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: LSD1 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human LSD1.
• Buffer: LSD1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: LSD1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LSD1 in P815 cell lysate with LSD1 antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of LSD1 in human breast carcinoma tissue with LSD1 antibody at 2 μg/ml.)

Immunofluorescence (IF)

(Immunofluorescence of LSD1 in human breast carcinoma tissue with LSD1 antibody at 20 μg/ml.Green: LSD1 Antibody (3761)Blue: DAPI staining)

Related Product Information for anti-KDM1 antibody

LSD1 Antibody: Histone modifications mediate changes in gene expression by altering chromatin structure or by serving as a platform to recruit other proteins. LSD1 is a recently discovered amine oxidase that catalyzes the lysine-specific demethylation of histone proteins via an FAD-dependent oxidative reaction. Methylation on histone H3-K9 is thought to play an important role in heterochromatin formation, while methylation on arginine and some lysine residues (such as H3-K4) is associated with active transcription. LSD1 associates with various proteins, including HDAC1/2, CoREST, and BHC80, that act to regulate LSD1 activity in vivo, and in a histone H3-K4-specific methylase complex that is involved in transcriptional regulation. Experiments have shown that CoREST, a SANT domain-containing corepressor acts to enhance LSD1 activity, while BHC80, a PHD domain-containing protein, inhibits CoREST/LSD1 activity in vitro. LSD1-mediated histone demethylation thus may have significant effects on gene expression.

NCBI and Uniprot Product Information

• NCBI GI #: 58761544
• NCBI GeneID: 23028
• NCBI Accession #: NP_001009999
• NCBI GenBank Nucleotide #: NM_001009999.2
• UniProt Accession #: O60341; Q5TH94; Q5TH95; Q86VT7; Q8IXK4; Q8NDP6; Q8TAZ3; Q96AW4; A8MWP9
• Molecular Weight: 95,155 Da
• NCBI Official Full Name: lysine-specific histone demethylase 1A isoform a
• NCBI Official Synonym Full Names: lysine (K)-specific demethylase 1A
• NCBI Official Symbol: KDM1A
• NCBI Official Synonym Symbols: AOF2; KDM1; LSD1; BHC110
• NCBI Protein Information: lysine-specific histone demethylase 1A; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; amine oxidase (flavin containing) domain 2; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-containing protein 2
• UniProt Protein Name: Lysine-specific histone demethylase 1A
• UniProt Gene Name: KDM1A
• UniProt Synonym Gene Names: AOF2; KDM1; KIAA0601; LSD1
• UniProt Entry Name: KDM1A_HUMAN

NCBI Description

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]

Uniprot Description

LSD1: Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr- 6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B. Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with ASXL1. Ubiquitously expressed. Belongs to the flavin monoamine oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; Nuclear receptor co-regulator; Oxidoreductase; EC 1.-.-.-; Demethylase

Chromosomal Location of Human Ortholog: 1p36.12

Cellular Component: nucleoplasm; transcription factor complex; nuclear chromatin; nucleus

Molecular Function: FAD binding; p53 binding; oxidoreductase activity; transcription factor binding; histone demethylase activity (H3-K9 specific); histone demethylase activity (H3-K4 specific); protein binding; MRF binding; enzyme binding; histone demethylase activity; androgen receptor binding; ligand-dependent nuclear receptor transcription coactivator activity; chromatin binding; transcription factor activity; demethylase activity

Biological Process: negative regulation of histone H3-K9 methylation; establishment and/or maintenance of chromatin architecture; transcription, DNA-dependent; granulocyte differentiation; in utero embryonic development; positive regulation of erythrocyte differentiation; negative regulation of transcription factor activity; muscle cell development; negative regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of DNA binding; protein amino acid demethylation; regulation of transcription from RNA polymerase II promoter; negative regulation of DNA damage response, signal transduction by p53 class mediator; cell proliferation; histone H3-K9 demethylation; pituitary gland development; positive regulation of hormone biosynthetic process; positive regulation of megakaryocyte differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; negative regulation of transcription, DNA-dependent; negative regulation of protein binding