Western Blot (WB)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate, Rat brain tissueAll lanes: LRTM2 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 kDaObserved band size: 42 kDa)
Rabbit anti-Human, Rat LRTM2 Polyclonal Antibody | anti-LRTM2 antibody
LRTM2 Antibody
Reactivity
Human, Rat
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
>95%Protein G Purified
Synonyms
LRTM2; Polyclonal Antibody; LRTM2 Antibody; Leucine-rich repeat and transmembrane domain-containing protein 2; anti-LRTM2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%
Protein G Purified
Protein G Purified
Form/Format
Liquid
Sequence Length
370
Applicable Applications for anti-LRTM2 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:1000-1:5000
IHC: 1:20-1:200
IF: 1:50-1:200
IHC: 1:20-1:200
IF: 1:50-1:200
Immunogen Species
Human
Immunogen
Recombinant Human Leucine-rich repeat and transmembrane domain-containing protein 2 protein (36-310AA)
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.
Western Blot (WB)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate, Rat brain tissueAll lanes: LRTM2 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 kDaObserved band size: 42 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: SH-SY5Y whole cell lysate, U251 whole cell lysate, Rat brain tissueAll lanes: LRTM2 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 42 kDaObserved band size: 42 kDa)
Immunohistochemistry (IHC)
(IHC image of CSB-PA847660LA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of CSB-PA847660LA01HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with CSB-PA847660LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Immunofluorescence (IF)
(Immunofluorescence staining of HepG2 cells with CSB-PA847660LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)
Product Categories/Family for anti-LRTM2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41,158 Da
NCBI Official Full Name
leucine-rich repeat and transmembrane domain-containing protein 2
NCBI Official Synonym Full Names
leucine rich repeats and transmembrane domains 2
NCBI Official Symbol
LRTM2
NCBI Protein Information
leucine-rich repeat and transmembrane domain-containing protein 2
UniProt Protein Name
Leucine-rich repeat and transmembrane domain-containing protein 2
UniProt Gene Name
LRTM2
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Product Notes
The LRTM2 lrtm2 (Catalog #AAA7114362) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LRTM2 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LRTM2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:1000-1:5000 IHC: 1:20-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the LRTM2 lrtm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LRTM2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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