Rabbit anti-Human SUSD5 Polyclonal Antibody | anti-SUSD5 antibody

SUSD5 Antibody

Cat. Num. AAA7113398

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: >95%; Protein G Purified
• Synonyms: SUSD5; Polyclonal Antibody; SUSD5 Antibody; Sushi domain-containing protein 5; KIAA0527; anti-SUSD5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >95%; Protein G Purified
• Form/Format: Liquid
• Sequence Length: 629

• Applicable Applications for anti-SUSD5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:1000-1:5000; IHC: 1:200-1:500; IF: 1:50-1:200
• Immunogen Species: Human
• Immunogen: Recombinant Human Sushi domain-containing protein 5 protein (46-287AA)
• Conjugate: Non-Conjugated
• Preservative: 0.03% Proclin 300
• Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
• Preparation and Storage: Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: U87 whole cell lysate, Hela whole cell lysateAll lanes: SUSD5 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 69 kDaObserved band size: 69 kDa)

Immunohistochemistry (IHC)

(IHC image of CSB-PA022971LA01HU diluted at 1:300 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescence staining of 293 cells with CSB-PA022971LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).)

Product Categories/Family for anti-SUSD5 antibody

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 150378552
• NCBI GeneID: 26032
• NCBI Accession #: NP_056366.1
• NCBI GenBank Nucleotide #: NM_015551.2
• UniProt Accession #: O60279
• Molecular Weight: 68,021 Da
• NCBI Official Full Name: sushi domain-containing protein 5
• NCBI Official Synonym Full Names: sushi domain containing 5
• NCBI Official Symbol: SUSD5
• NCBI Protein Information: sushi domain-containing protein 5
• UniProt Protein Name: Sushi domain-containing protein 5
• Protein Family: Sushi domain-containing protein
• UniProt Gene Name: SUSD5
• UniProt Synonym Gene Names: KIAA0527