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Western Blot (WB) (Western BlotPositive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysate, U251 whole cell lysateAll lanes: AHI1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 138, 121, 71 kDaObserved band size: 138 kDa)

Rabbit anti-Human AHI1 Polyclonal Antibody | anti-AHI1 antibody

AHI1 Antibody

Gene Names
AHI1; ORF1; AHI-1; JBTS3; dJ71N10.1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
>95%
Protein G Purified
Synonyms
AHI1; Polyclonal Antibody; AHI1 Antibody; Jouberin; Abelson helper integration site 1 protein homolog; AHI-1; anti-AHI1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
>95%
Protein G Purified
Form/Format
Liquid
Sequence Length
1196
Applicable Applications for anti-AHI1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1000-1:5000
IHC: 1:200-1:500
Immunogen Species
Human
Immunogen
Recombinant Human Jouberin protein (1-213AA)
Conjugate
Non-Conjugated
Preservative
0.03% Proclin 300
Constituents
50% Glycerol, 0.01M PBS, pH 7.4
Preparation and Storage
Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysate, U251 whole cell lysateAll lanes: AHI1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 138, 121, 71 kDaObserved band size: 138 kDa)

Western Blot (WB) (Western BlotPositive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate, HL-60 whole cell lysate, U251 whole cell lysateAll lanes: AHI1 antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 138, 121, 71 kDaObserved band size: 138 kDa)

Immunohistochemistry (IHC)

(IHC image of CSB-PA836642LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC) (IHC image of CSB-PA836642LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Product Categories/Family for anti-AHI1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
120,853 Da
NCBI Official Full Name
jouberin isoform a
NCBI Official Synonym Full Names
Abelson helper integration site 1
NCBI Official Symbol
AHI1
NCBI Official Synonym Symbols
ORF1; AHI-1; JBTS3; dJ71N10.1
NCBI Protein Information
jouberin
UniProt Protein Name
Jouberin
Protein Family
UniProt Gene Name
AHI1
UniProt Synonym Gene Names
AHI-1

NCBI Description

This gene is apparently required for both cerebellar and cortical development in humans. This gene mutations cause specific forms of Joubert syndrome-related disorders. Joubert syndrome (JS) is a recessively inherited developmental brain disorder with several identified causative chromosomal loci. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Oct 2008]

Uniprot Description

Involved in vesicle trafficking and required for ciliogenesis, formation of primary non-motile cilium, and recruitment of RAB8A to the basal body of primary cilium. Component of the tectonic-like complex, a complex localized at the transition zone of primary cilia and acting as a barrier that prevents diffusion of transmembrane proteins between the cilia and plasma membranes. Involved in neuronal differentiation. As a positive modulator of classical Wnt signaling, may play a crucial role in ciliary signaling during cerebellum embryonic development (PubMed:21623382).

Research Articles on AHI1

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Product Notes

The AHI1 ahi1 (Catalog #AAA7114274) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AHI1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AHI1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1000-1:5000 IHC: 1:200-1:500. Researchers should empirically determine the suitability of the AHI1 ahi1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AHI1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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