Goat LRRK2/PARK8 Polyclonal Antibody | anti-LRRK2 antibody

Goat anti-LRRK2/PARK8 (near C Terminus) Antibody

Cat. Num. AAA420395

• Gene Names: LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Cow
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: LRRK2/PARK8; Polyclonal Antibody; Goat anti-LRRK2/PARK8 (near C Terminus) Antibody; LRRK2; PARK8; ROCO2; FLJ45829; DKFZp434H2111; leucine-rich repeat kinase 2; dardarin; Parkinson disease (autosomal dominant) 8; AURA17; DARDARIN; RIPK7; augmented in rheumatoid arthritis 17; LRRK2 antibody; PARK8 antibody; ROCO2 antibody; FLJ45829 antibody; DKFZp434H2111 antibody; leucine-rich repeat kinase 2 antibody; dardarin antibody; Parkinson disease (autosomal dominant) 8 antibody; LRRK2 / PARK8 (near C Terminus); anti-LRRK2 antibody

• Host: Goat
• Reactivity: Tested: Human; Expected from sequence similarity: Human, Cow
• Clonality: Polyclonal
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Concentration: 100ug specific antibody in 200ul (varies by lot)
• Sequence: CELAEKMRRTSV
• Sequence Length: 2527

• Applicable Applications for anti-LRRK2 antibody: Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoelectron Microscopy (IEM)
• Application Notes: Peptide ELISA: Antibody detection limit dilution 1: 64000.; Immunohistochemistry: In paraffin embedded Human Brain (hippocampus) shows staining of neuronal cytoplasm. Recommended concentration, 1-2ug/ml. This product has been successfully used in immunohistochemistry on human brain: Alegre-Abarrategui J et al., Neuropathol Appl Neurobiol.2007 Oct 26; PMID: 17971075; Western Blot: This product has been successfully used in Western blot for human brain as described in the following paper: Sharma et al, Neuropathol Appl Neurobiol.2011 Jun 23, PMID 21696411. Recommended concentration: 0.3-1ug/ml.; Immunoelectron microscopy: This antibody has been successfully used in IEM as described in the following paper: Alegre-Abarrategui et al, Hum Mol Genet.2009 Nov 1;18(21): 4022-34, PMID: 19640926.; ICC: This antibody has also been successfully used in ICC for human neurons as described in: Sharma et al, Neuropathol Appl Neurobiol.2011 Jun 23, PMID 21696411.
• Immunogen: Peptide with sequence CELAEKMRRTSV, from the internal region (near the C Terminus) of the protein sequence according to NP_940980.3.
• Epitope: Internal region (near the C Terminus)
• Preparation and Storage: Aliquot and store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

((1.5ug/ml) staining of paraffin embedded Human Hippocampus CA4. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining.)

NCBI and Uniprot Product Information

• NCBI GI #: 171846278
• NCBI GeneID: 120892
• NCBI Accession #: NP_940980.3
• NCBI GenBank Nucleotide #: NM_198578.3
• Molecular Weight: 286,103 Da
• NCBI Official Full Name: leucine-rich repeat serine/threonine-protein kinase 2
• NCBI Official Synonym Full Names: leucine-rich repeat kinase 2
• NCBI Official Symbol: LRRK2
• NCBI Official Synonym Symbols: PARK8; RIPK7; ROCO2; AURA17; DARDARIN
• NCBI Protein Information: leucine-rich repeat serine/threonine-protein kinase 2
• UniProt Protein Name: Leucine-rich repeat serine/threonine-protein kinase 2
• Protein Family: Leucine-rich repeat serine/threonine-protein kinase
• UniProt Gene Name: LRRK2
• UniProt Synonym Gene Names: PARK8
• UniProt Entry Name: LRRK2_HUMAN

NCBI Description

This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]

Uniprot Description

LRRK2: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). May play a role in the etiology of Parkinson disease. May also have GTPase activity. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Interacts with PARK2, PRDX3 and TPCN2. Expressed throughout the adult brain, but at a lower level than in heart and liver. Expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.

Protein type: Kinase, protein; Protein kinase, TKL; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; LRRK family

Chromosomal Location of Human Ortholog: 12q12

Cellular Component: axon; caveola; cell junction; cell soma; cytoplasm; cytoplasmic vesicle; cytosol; dendrite; dendrite cytoplasm; endoplasmic reticulum; endosome; extracellular space; Golgi apparatus; Golgi-associated vesicle; growth cone; inclusion body; intracellular; lysosome; microvillus; mitochondrial inner membrane; mitochondrial matrix; mitochondrial membrane; mitochondrial outer membrane; mitochondrion; neuron projection; perikaryon; plasma membrane; ribonucleoprotein complex; synaptic vesicle membrane; terminal button; trans-Golgi network

Molecular Function: actin binding; ATP binding; clathrin binding; glycoprotein binding; GTP binding; GTP-dependent protein kinase activity; GTPase activator activity; GTPase activity; identical protein binding; kinase activity; MAP kinase kinase activity; microtubule binding; protein binding; protein homodimerization activity; protein kinase A binding; protein kinase activity; protein serine/threonine kinase activity; receptor signaling complex scaffold activity; Rho GTPase binding; SNARE binding; syntaxin-1 binding; tubulin binding

Biological Process: activation of MAPK activity; activation of MAPKK activity; autophagy; calcium-mediated signaling; cellular response to starvation; determination of adult life span; endocytosis; Golgi organization and biogenesis; GTP metabolic process; intracellular distribution of mitochondria; lysosome organization and biogenesis; MAPKKK cascade; mitochondrion localization; mitochondrion organization and biogenesis; negative regulation of macroautophagy; negative regulation of protein amino acid phosphorylation; negative regulation of protein binding; neurite morphogenesis; neuromuscular junction development; olfactory bulb development; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of autophagy; positive regulation of dopamine receptor signaling pathway; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of programmed cell death; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of protein ubiquitination; protein amino acid autophosphorylation; protein amino acid phosphorylation; protein transport; regulation of autophagy; regulation of calcium-mediated signaling; regulation of dopamine receptor signaling pathway; regulation of excitatory postsynaptic membrane potential; regulation of locomotion; regulation of membrane potential; regulation of mitochondrial depolarization; regulation of neuron maturation; regulation of synaptic transmission, glutamatergic; response to oxidative stress; small GTPase mediated signal transduction; tangential migration from the subventricular zone to the olfactory bulb; Wnt receptor signaling pathway through beta-catenin

Disease: Parkinson Disease 8, Autosomal Dominant